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Rabbit Recombinant Monoclonal GABA A Receptor alpha 5/GABRA5 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.

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Images

Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (AB259880), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (AB259880), expandable thumbnail
  • Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (AB259880), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (AB259880), expandable thumbnail
  • Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (AB259880), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-FrIHC-P
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Tested
Tested
Not recommended
Tested
Rat
Not recommended
Tested
Tested
Not recommended
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Rat
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:14993607). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (By similarity). GABAARs containing alpha-5/GABRA5 are mainly extrasynaptic and contribute to the tonic GABAergic inhibition of the hippocampus (PubMed:14993607). Extrasynaptic alpha-5-containing GABAARs in CA1 pyramidal neurons play a role in learning and memory processes (PubMed:14993607).

Alternative names

Gamma-aminobutyric acid receptor subunit alpha-5, GABA(A) receptor subunit alpha-5, GABAAR subunit alpha-5, Gabra5

Recommended products

Rabbit Recombinant Monoclonal GABA A Receptor alpha 5/GABRA5 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24003-118
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880), expandable thumbnail

    Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880)

    GABA A Receptor alpha 5/GABRA5 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab259880 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259880 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab259880 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259880 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 93 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880)

    Predicted band size: 52 kDa

    Observed band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labelling GABA A Receptor alpha 5/GABRA5 with ab259880 at 1/50 (11.4 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse hippocampus (PMID:27364953). The section was incubated with ab259880 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880), expandable thumbnail

    Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880)

    GABA A Receptor alpha 5/GABRA5 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab259880 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259880 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10 ug

    Lane 2: ab259880 IP in rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259880 in rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 93 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880)

    Predicted band size: 52 kDa

    Observed band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880)

    Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labelling GABA A Receptor alpha 5/GABRA5 with ab259880 at 1/50 (11.4 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat hippocampus. The section was incubated with ab259880 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880), expandable thumbnail

    Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse hippocampus tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa

    Observed band size: 55 kDa

  • Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880), expandable thumbnail

    Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880) at 1/1000 dilution

    All lanes: Rat hippocampus tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa

    Observed band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling GABA A Receptor alpha 5/GABRA5 with ab259880 at 1/50 (11.4 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: almost no staining on mouse spleen. The section was incubated with ab259880 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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