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Mouse Recombinant Monoclonal GABA A Receptor alpha 5/GABRA5 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples.

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Images

Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (AB302962), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (AB302962), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (AB302962), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (AB302962), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (AB302962), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Tested
Not recommended
Rat
Tested
Tested
Not recommended

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:14993607). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (By similarity). GABAARs containing alpha-5/GABRA5 are mainly extrasynaptic and contribute to the tonic GABAergic inhibition of the hippocampus (PubMed:14993607). Extrasynaptic alpha-5-containing GABAARs in CA1 pyramidal neurons play a role in learning and memory processes (PubMed:14993607).

Alternative names

Gamma-aminobutyric acid receptor subunit alpha-5, GABA(A) receptor subunit alpha-5, GABAAR subunit alpha-5, Gabra5

Recommended products

Mouse Recombinant Monoclonal GABA A Receptor alpha 5/GABRA5 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
N415--24
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962), expandable thumbnail

    Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: spleen (human protein atlas database). Samples are non-boiled as boiling may cause protein aggregates. There is an unknown band at 130 kDa.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962) at 1/1000 dilution

    Lane 1: Rat hippocampus tissue lysate at 40 µg

    Lane 2: Rat spleen tissue lysate at 40 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

    Developed using the ECL technique.

    Observed band size: 55 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue to label GABA A Receptor alpha 5/GABRA5 with ab302962 at 1/200 (5.59 μg/ml) followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the Leica DS9800 kit staining procedure.

    Negative control: no staining on rat spleen.

    The section was incubated with ab302962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue to label GABA A Receptor alpha 5/GABRA5 with ab302962 at 1/200 (5.59 μg/ml) followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the Leica DS9800 kit staining procedure.

    Negative control: no staining on mouse spleen.

    The section was incubated with ab302962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling GABA A Receptor alpha 5/GABRA5 with ab302962 at 1/200 (5.59 μg/ml) followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the Leica DS9800 kit staining procedure. Positive staining on rat hippocampus. The section was incubated with ab302962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (ab302962)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling GABA A Receptor alpha 5/GABRA5 with ab302962 at 1/200 (5.59 μg/ml) followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the Leica DS9800 kit staining procedure. Positive staining on mouse hippocampus. The section was incubated with ab302962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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