Mouse Recombinant Monoclonal GABA A Receptor alpha 5/GABRA5 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse samples.
Images
![Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (AB302963), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-alpha-5-gabra5-antibody-n415-24-bsa-and-azide-free-ab302963--western-blot-img404498.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (AB302963)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (AB302963), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-alpha-5-gabra5-antibody-n415-24-bsa-and-azide-free-ab302963--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403990.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (AB302963)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (AB302963), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-alpha-5-gabra5-antibody-n415-24-bsa-and-azide-free-ab302963--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403989.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (AB302963)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (AB302963), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-alpha-5-gabra5-antibody-n415-24-bsa-and-azide-free-ab302963--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403988.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (AB302963)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (AB302963), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-alpha-5-gabra5-antibody-n415-24-bsa-and-azide-free-ab302963--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403987.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (AB302963)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended |
| Rat | Tested | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:14993607). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (By similarity). GABAARs containing alpha-5/GABRA5 are mainly extrasynaptic and contribute to the tonic GABAergic inhibition of the hippocampus (PubMed:14993607). Extrasynaptic alpha-5-containing GABAARs in CA1 pyramidal neurons play a role in learning and memory processes (PubMed:14993607).
Alternative names
Gamma-aminobutyric acid receptor subunit alpha-5, GABA(A) receptor subunit alpha-5, GABAAR subunit alpha-5, Gabra5
Recommended products
Mouse Recombinant Monoclonal GABA A Receptor alpha 5/GABRA5 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- N415--24
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab302963 is a carrier version of Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-alpha-5-gabra5-antibody-n415-24-bsa-and-azide-free-ab302963--western-blot-img404498.jpg "Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963)")
Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963)
This data was developed using Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: spleen (human protein atlas database). Samples are non-boiled as boiling may cause protein aggregates. There is an unknown band at 130 kDa.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] (Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962) at 1/1000 dilution
Lane 1: Rat hippocampus tissue lysate at 40 µg
Lane 2: Rat spleen tissue lysate at 40 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 55 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-alpha-5-gabra5-antibody-n415-24-bsa-and-azide-free-ab302963--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403990.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963)
This data was developed using Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue to label GABA A Receptor alpha 5/GABRA5 with Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962 at 1/200 (5.59 μg/ml) followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the Leica DS9800 kit staining procedure.
Negative control: no staining on rat spleen.
The section was incubated with Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-alpha-5-gabra5-antibody-n415-24-bsa-and-azide-free-ab302963--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403989.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963)
This data was developed using Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962, the same antibody clone in a different buffer formulation
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue to label GABA A Receptor alpha 5/GABRA5 with Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962 at 1/200 (5.59 μg/ml) followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the Leica DS9800 kit staining procedure.
Negative control: no staining on mouse spleen.
The section was incubated with Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-alpha-5-gabra5-antibody-n415-24-bsa-and-azide-free-ab302963--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403988.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963)
This data was developed using Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling GABA A Receptor alpha 5/GABRA5 with Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962 at 1/200 (5.59 μg/ml) followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the Leica DS9800 kit staining procedure. Positive staining on rat hippocampus. The section was incubated with Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-alpha-5-gabra5-antibody-n415-24-bsa-and-azide-free-ab302963--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403987.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] - BSA and Azide Free (ab302963)
This data was developed using Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling GABA A Receptor alpha 5/GABRA5 with Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962 at 1/200 (5.59 μg/ml) followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the Leica DS9800 kit staining procedure. Positive staining on mouse hippocampus. The section was incubated with Anti-GABA A Receptor alpha 5/GABRA5 antibody [N415/24] ab302962 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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