AB242001

Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24]

Name: Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (ab242001) | Abcam
Brand: Abcam Limited
SKU: AB242001
Price: 520 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal GABA A Receptor alpha 5/GABRA5 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GABRA5 aa 350-450.

View Alternative Names

Gamma-aminobutyric acid receptor subunit alpha-5, GABA(A) receptor subunit alpha-5, GABAAR subunit alpha-5, GABRA5

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (AB242001)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (AB242001)

4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for GABA A Receptor alpha 5/GABRA5 (green) using ab242001 at 1/100 dilution for 1 hour at room temperature in ICC. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes at room temperature. Actin is detected with Phalloidin Texas Red F-Actin stain (red). The nuclear counterstain is DAPI (blue).

Supplier Data

Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (AB242001)

Primary incubation for 16 hours at 4°C.

Secondary incubation for 1 hr at RT.

All lanes:

Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (ab242001) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/200 dilution

Predicted band size: 52 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

S415-24

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GABRA5 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

P31644

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed : 14993607, PubMed : 29961870, PubMed : 30140029, PubMed : 31056671). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed : 30140029). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed : 14993607, PubMed : 30140029). GABAARs containing alpha-5/GABRA5 subunits are mainly extrasynaptic and contribute to the tonic GABAergic inhibition in the hippocampus (By similarity). Extrasynaptic alpha-5-containing GABAARs in CA1 pyramidal neurons play a role in learning and memory processes (By similarity).

See full target information GABRA5

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 23:e14209 PubMed38825816

2024

Sevoflurane-induced overexpression of extrasynaptic α5-GABAR via the RhoA/ROCK2 pathway impairs cognitive function in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhun Wang,Jinpeng Dong,Mengxue Zhang,Sixuan Wang,Jiangnan Wu,Shengran Wang,Yuan Luo,Yongan Wang,Yiqing Yin

Immunity, inflammation and disease 12:e1194 PubMed38501544

2024

Sodium butyrate activates the K channels to regulate the mechanism of Parkinson's disease microglia model inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Xu,Laofu Wen,Yunyi Tang,Zhenqiang Zhao,Miaojing Xu,Tan Wang,Zhibin Chen

Journal of neuroinflammation 18:204 PubMed34530841

2021

MD2 contributes to the pathogenesis of perioperative neurocognitive disorder via the regulation of α5GABA receptors in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqiang Zuo,Jianshuai Zhao,Jinming Zhang,Zongping Fang,Jiao Deng,Ze Fan,Yaru Guo,Jing Han,Wugang Hou,Hailong Dong,Feifei Xu,Lize Xiong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24]

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (AB242001)

Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (AB242001)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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