Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)

Rabbit Monoclonal GABA A Receptor alpha 6 antibody. Carrier free. Suitable for mIHC, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (AB300070), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	WB	IHC-Fr	ICC/IF	Flow Cyt (Intra)	IP	IHC-P
Human	Tested	Tested	Expected	Not recommended	Not recommended	Not recommended	Expected
Mouse	Tested	Tested	Tested	Not recommended	Not recommended	Not recommended	Tested
Rat	Expected	Tested	Tested	Not recommended	Not recommended	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information GABRA6

Function

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:8632757). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (By similarity). Alpha-6/GABRA6 subunits are found at both synaptic and extrasynaptic sites (PubMed:8632757). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (By similarity). Extrasynaptic alpha-6-containing receptors contribute to the tonic GABAergic inhibition. Alpha-6 subunits are also present on glutamatergic synapses (By similarity).

Alternative names

Gamma-aminobutyric acid receptor subunit alpha-6, GABA(A) receptor subunit alpha-6, GABAAR subunit alpha-6, GABRA6

Recommended products

Rabbit Monoclonal GABA A Receptor alpha 6 antibody. Carrier free. Suitable for mIHC, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR25322-129
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Aliquoting information: Upon delivery aliquot

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)
This data was developed using Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069, the same antibody clone in a different buffer formulation.
5% NFDM/TBST was used as blocking and diluting buffer.
Low expression：Heart (PMID:29467616).
All lanes: Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069) at 1/1000 dilution
Lane 1: Human hypothalamus tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 57 kDa
Exposure time: 180s
Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)
This data was developed using Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069, the same antibody clone in a different buffer formulation.
5% NFDM/TBST was used as blocking and diluting buffer.
All lanes: Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069) at 1/1000 dilution
All lanes: Mouse brain tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 57 kDa
Exposure time: 59s
Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)
This data was developed using Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Heart (PMID:29467616)
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Rat cerebellum tissue lysate at 20 µg
Lane 3: Rat heart tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 57 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)
This data was developed using Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling GABA A Receptor alpha 6 with Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069 at 1/500 dilution (1.244 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cerebellum. The section was incubated with Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)
This data was developed using Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labelling GABA A Receptor alpha 6 with Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069 at 1/500 dilution (1.244 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cerebellum. The section was incubated with Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)
This data was developed using Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labelling GABA A Receptor alpha 6 with Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069 at 1/500 dilution (1.244 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on human cerebellum. The section was incubated with Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)
This data was developed using Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling GABA A Receptor alpha 6 with Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069 at 1/500 (1.244 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 μ/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)
This data was developed using Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling GABA A Receptor alpha 6 with Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069 at 1/500 (1.244 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 μ/mL) dilution.
Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)
This data was developed using Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: heart (PMID:29467616).
This blot was developed using a high ECL substrate.
Sometimes a non-specific band near the target signal can be observed.
All lanes: Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Human hypothalamus tissue lysate at 20 µg
Lane 3: Human heart tissue lysate at 20 µg
Lane 4: Mouse brain tissue lysate at 20 µg
Lane 5: Mouse cerebellum tissue lysate at 20 µg
Lane 6: Mouse heart tissue lysate at 20 µg
Lane 7: Rat brain tissue lysate at 20 µg
Lane 8: Rat cerebellum tissue lysate at 20 µg
Lane 9: Rat heart tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP), minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 57 kDa
Exposure time: 180s
Multiplex immunohistochemistry - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] - BSA and Azide free (ab300070)
Fluorescence multiplex immunohistochemical analysis of mouse cerebellum (formalin-fixed paraffin-embedded section).

Panel A: merged staining of anti-GABRA6 (magenta; Opal™690), anti-KCNA (green; Opal™520) and anti-DGKZ (gray; Opal™570) on mouse cerebellum.

Panel B: anti-GABRA6 staining granule cells in mouse cerebellum.

Panel C: anti-KCNA staining basket cells in mouse cerebellum.

Panel D: anti-DGKZ staining Purkinje cells in mouse cerebellum.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] ab300069 at a 1/500 dilution, Anti-KCNA antibody [EPR26383-85] ab313624 at a 1/5000 and Anti-DGKZ/DGK-zeta antibody [EPR22040-72] ab239080 at a 1/5000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.