Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free (AB309541)

This data was developed using ab309540, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GABA A Receptor beta 2 with ab309540 at 1/100 (5.12 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : No staining on human pancreas. The section was incubated with ab309540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free (AB309541)

This data was developed using ab309540, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GABA A Receptor beta 2 with ab309540 at 1/100 (5.12 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive on human cerebrum. The section was incubated with ab309540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free (AB309541)

This data was developed using ab309540, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GABA A Receptor beta 2 with ab309540 at 1/2000 (0.256 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive on mouse cerebrum.The section was incubated with ab309540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free (AB309541)

This data was developed using ab309540, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling GABA A Receptor beta 2 with ab309540 at 1/2000 (5.12 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : No staining on rat skeletal muscle. The section was incubated with ab309540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free (AB309541)

This data was developed using ab309540, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GABA A Receptor beta 2 with ab309540 at 1/2000 (0.256 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive on rat cerebrum.The section was incubated with ab309540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free (AB309541)

This data was developed using ab309540, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling GABA A Receptor beta 2 with ab309540 at 1/2000 (5.12 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : No staining on mouse skeletal muscle. The section was incubated with ab309540 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free (AB309541)

This data was developed using ab309540, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling GABA A Receptor beta 2 with ab309540 at 1/50 (10.24 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) pre-adsorbed at 1/1000 2 ug/mL dilution (Green). Panel A : merged staining of anti-GABRB2 (ab309540, green), anti-NeuN (ab190565, grey) and anti-GFAP (ab201732, red) on rat cerebellum. Panel B : anti-GABRB2 stained on rat cerebellum. Panel C : anti-NeuN stained in neurons of rat cerebellum. Panel D : anti-GFAP stained in astrocytes of rat cerebellum. The section was incubated in two rounds of staining : in the order of ab309540 and ab190565, ab201732 for 1 hour at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope. is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) pre-adsorbed at 1/1000 2 ug/mL dilution.<\p> <\p>

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free (AB309541)

This data was developed using ab309540, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh) tissue labeling GABA A Receptor beta 2 with ab309540 at 1/50 (10.24 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluo® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab309540 for 60 mins at room temperature. The section was then mounted using Fluoromount. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope. is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed at 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free (AB309541)

This data was developed using ab309540, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling GABA A Receptor beta 2 with ab309540 at 1/50 (10.24 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Panel A : merged staining of anti-GABRB2 (ab309540, green), anti-NeuN (ab190565, grey) and anti-GFAP (ab201732, red) on mouse cerebellum.Panel B : anti-GABRB2 stained on mouse cerebellum.Panel C : anti-NeuN stained in neurons of mouse cerebellum.Panel D : anti-GFAP stained in astrocytes of mouse cerebellum.The section was incubated in two rounds of staining : in the order of ab309540 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> <\p>

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor beta 2 antibody [EPR28366-42] - BSA and Azide free (AB309541)

This data was developed using ab309540, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh) tissue labeling GABA A Receptor beta 2 with ab309540 at 1/50 (10.24 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) pre-adsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on Rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab309540 for 60 mins at room temperature. The section was then mounted using Fluoromount. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) pre-adsorbed at 1/1000 2 ug/mL dilution.