AB156000

Anti-GABA A Receptor beta 2/GABRB2 antibody [EPR10517]

RabMAb
Recombinant
What is this?

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(14 Publications )

Rabbit Recombinant Monoclonal GABA A Receptor beta 2/GABRB2 antibody. Suitable for WB and reacts with Human samples. Cited in 14 publications.

View Alternative Names

Gamma-aminobutyric acid receptor subunit beta-2, GABA(A) receptor subunit beta-2, GABAAR subunit beta-2, GABRB2

1 Images

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Western blot - Anti-GABA A Receptor beta 2/GABRB2 antibody [EPR10517] (AB156000)

All lanes:

Western blot - Anti-GABA A Receptor beta 2/GABRB2 antibody [EPR10517] (ab156000) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

Fetal brain lysate at 10 µg

Lane 4:

Human cerebellum lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 59 kDa

false

Carrier free

Anti-GABA A Receptor beta 2/GABRB2 antibody [EPR10517] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10517

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Beta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed : 19763268, PubMed : 27789573, PubMed : 29950725, PubMed : 8264558). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed : 29950725). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (By similarity). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (By similarity). GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation (PubMed : 23909897, PubMed : 25489750). Extrasynaptic beta-2 receptors contribute to the tonic GABAergic inhibition (By similarity). Beta-containing GABAARs can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness (By similarity).

See full target information GABRB2

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 15:4872-4885 PubMed41049747

2025

The toxic components, toxicological mechanism and effective antidote for poisoning.

Applications

Unspecified application

Species

Unspecified reactive species

Niping Li,Yaorong Yang,Shengyuan Zhang,Bin Jiang,Wei Zhang,Haibo Wang,Lixin Chen,Liwei Wang,Yiyi Li,Lei Shi,Wencai Ye,Lei Wang

Journal of neurochemistry 164:786-812 PubMed36695027

2023

Translational patterns of ionotropic glutamate and GABA receptors during brain development and behavioral stimuli revealed by polysome profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Peng,Miaoqi Huang,Jiaoyan Xie,Beiyi Shu,Xiaojun Wang,Yumei Liao,Junyu Xu,Lei Shi

Aging cell 21:e13573 PubMed35199454

2022

Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Shen,Xiaolei Wu,Zhan Zhang,Di Zhang,Sihua Yang,Da Xing

Molecular neurodegeneration 16:14 PubMed33663578

2021

Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Chen,Yunan Chen,Qingwei Huo,Lei Wang,Shuyi Tan,Afzal Misrani,Jinxiang Jiang,Jian Chen,Shiyuan Chen,Jiawei Zhang,Sidra Tabassum,Jichen Wang,Xi Chen,Cheng Long,Li Yang

International journal of molecular sciences 21: PubMed33291462

2020

Sleep/Wake Behavior and EEG Signatures of the TgF344-AD Rat Model at the Prodromal Stage.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Kreuzer,Glenda L Keating,Thomas Fenzl,Lorenz Härtner,Christopher G Sinon,Ihab Hajjar,Vincent Ciavatta,David B Rye,Paul S García

Anesthesia and analgesia 129:192-203 PubMed31082969

2019

Chronic Calcineurin Inhibition via Cyclosporine A Impairs Visuospatial Learning After Isoflurane Anesthesia.

Applications

Unspecified application

Species

Unspecified reactive species

Iris A Speigel,Christopher M Ma,Edyta K Bichler,Jennifer L Gooch,Paul S García

The Journal of clinical investigation 129:2333-2350 PubMed31063987

2019

CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Zhang,Qi Yang,Le Yang,Yan-Jiao Li,Xin-Shang Wang,Yu-Jiao Li,Rui-Li Dang,Shao-Yu Guan,Yan-Yan Guo,Ting Sun,Yu-Mei Wu,An Liu,Yan Zhang,Shui-Bing Liu,Ming-Gao Zhao

Molecular pain 14:1744806918783478 PubMed29956582

2018

Chronic inflammatory pain induced GABAergic synaptic plasticity in the adult mouse anterior cingulate cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Kohei Koga,Shuji Shimoyama,Akihiro Yamada,Tomonori Furukawa,Yoshikazu Nikaido,Hidemasa Furue,Kazuhiko Nakamura,Shinya Ueno

Journal of neuropathology and experimental neurology 77:608-627 PubMed29788265

2018

Gene Expression Profile in Frontal Cortex in Sporadic Frontotemporal Lobar Degeneration-TDP.

Applications

Unspecified application

Species

Unspecified reactive species

Pol Andrés-Benito,Ellen Gelpi,Mónica Povedano,Gabriel Santpere,Isidro Ferrer

eLife 6: PubMed28054918

2017

APP modulates KCC2 expression and function in hippocampal GABAergic inhibition.

Applications

Species

Mouse

Ming Chen,Jinzhao Wang,Jinxiang Jiang,Xingzhi Zheng,Nicholas J Justice,Kun Wang,Xiangqian Ran,Yi Li,Qingwei Huo,Jiajia Zhang,Hongmei Li,Nannan Lu,Ying Wang,Hui Zheng,Cheng Long,Li Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABA A Receptor beta 2/GABRB2 antibody [EPR10517]

Western blot - Anti-GABA A Receptor beta 2/GABRB2 antibody [EPR10517] (AB156000)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (14)

Product promise