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Rabbit Monoclonal GABA A Receptor beta 3/GABRB3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064), expandable thumbnail
  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064), expandable thumbnail
  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064), expandable thumbnail
  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (AB300064), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-PFlow Cyt (Intra)IPIHC-Fr
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Target data

Function

Beta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:14993607, PubMed:18514161, PubMed:22243422, PubMed:22303015, PubMed:24909990, PubMed:26950270, PubMed:30602789). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed:24909990, PubMed:30140029, PubMed:30602789). GABAARs containing beta-3/GABRB3 subunit are found at both synaptic and extrasynaptic sites (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed:14993607, PubMed:22303015, PubMed:26950270, PubMed:30602789). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (PubMed:22303015, PubMed:26950270). GABAARs containing alpha-1 and beta-3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation (PubMed:25489750). Extrasynaptic beta-3 receptors contribute to the tonic GABAergic inhibition (By similarity). GABAARs containing alpha-1, beta-3 and epsilon subunits may also permit spontaneous chloride channel activity while preserving the structural information required for GABA-gated openings (By similarity). Beta-containing GABAARs can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness (PubMed:18281286, PubMed:24909990, PubMed:35355020). Plays an important role in somatosensation and in the production of antinociception (By similarity).

Alternative names

Gamma-aminobutyric acid receptor subunit beta-3, GABA(A) receptor subunit beta-3, GABAAR subunit beta-3, GABRB3

Recommended products

Rabbit Monoclonal GABA A Receptor beta 3/GABRB3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR25323-127
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (ab300064), expandable thumbnail

    Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (ab300064)

    This data was developed using Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] ab300063, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as blocking and diluting buffer and concentration.

    Samples are non-boiled as boiling may cause protein aggregates.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 22303015 ).
    Low expression: HEK-293(PMID:22303015 ), U937 (HPA).

    All lanes: Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] ab300063) at 1/1000 dilution

    Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa

    Observed band size: 43-54 kDa

    Exposure time: 48s

  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (ab300064), expandable thumbnail

    Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (ab300064)

    This data was developed using Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] ab300063, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as blocking and diluting buffer and concentration.

    Samples are non-boiled as boiling may cause protein aggregates.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID:22303015).

    All lanes: Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] ab300063) at 1/1000 dilution

    Lane 1: Mouse cerebellum tissue lysate at 20 µg

    Lane 2: Mouse hippocampus tissue lysate at 20 µg

    Lane 3: Rat cerebellum tissue lysate at 20 µg

    Lane 4: Rat hippocampus tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa

    Observed band size: 43-54 kDa

    Exposure time: 26s

  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (ab300064), expandable thumbnail

    Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (ab300064)

    This data was developed using Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] ab300063, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as blocking and diluting buffer and concentration.

    Samples are non-boiled as boiling may cause protein aggregates.
    Lysates should be made freshly and used in WB immediately to minimize protein degradation.

    All lanes: Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] ab300063) at 1/1000 dilution

    All lanes: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa

    Observed band size: 54 kDa

    Exposure time: 180s

  • Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (ab300064), expandable thumbnail

    Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (BSA and Azide free) (ab300064)

    This data was developed using Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] ab300063, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as blocking and diluting buffer and concentration.

    Samples are non-boiled as boiling may cause protein aggregates.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID:22303015).

    All lanes: Western blot - Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] (Anti-GABA A Receptor beta 3/GABRB3 antibody [EPR25323-127] ab300063) at 1/1000 dilution

    All lanes: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa

    Observed band size: 43-54 kDa

    Exposure time: 48s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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