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AB307231

Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69]

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(1 Publication)

Mouse Recombinant Monoclonal GABA A Receptor gamma 2/GABRG2 antibody. Suitable for IHC-P and reacts with Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, GABAAR subunit gamma-2, GABRG2

3 Images
Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] (AB307231)
  • IHC

Supplier Data

Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] (AB307231)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling GABA A Receptor gamma 2/GABRG2 with ab307231 at 1/500 dilution (1.654 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse kidney. The section was incubated with ab307231 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] (AB307231)
  • IHC

Supplier Data

Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] (AB307231)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling GABA A Receptor gamma 2/GABRG2 with ab307231 at 1/500 dilution (1.654 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with ab307231 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] (AB307231)
  • IHC

Supplier Data

Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] (AB307231)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling GABA A Receptor gamma 2/GABRG2 with ab307231 at 1/500 dilution (1.654 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum is observed. The section was incubated with ab307231 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Carrier free

    Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N452/69

Isotype

IgG2a

Carrier free

No

Reacts with

Rat, Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Gamma subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed : 14993607, PubMed : 16412217, PubMed : 23909897, PubMed : 2538761, PubMed : 25489750, PubMed : 27864268, PubMed : 29950725, PubMed : 30602789). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed : 29950725, PubMed : 30602789). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed : 14993607, PubMed : 16412217, PubMed : 2538761, PubMed : 27864268, PubMed : 29950725, PubMed : 30602789). Gamma-2/GABRG2-containing GABAARs are found at both synaptic and extrasynaptic sites (By similarity). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (By similarity). GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation (PubMed : 23909897, PubMed : 25489750). Extrasynaptic gamma-2-containing receptors contribute to the tonic GABAergic inhibition (By similarity). GABAARs function also as histamine receptor where histamine binds at the interface of two neighboring beta subunits and potentiates GABA response in a gamma-2 subunit-controlled manner (By similarity).
See full target information GABRG2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:728 PubMed38877285

2024

An ultra-short-acting benzodiazepine in thalamic nucleus reuniens undermines fear extinction via intermediation of hippocamposeptal circuits.

Applications

Unspecified application

Species

Unspecified reactive species

Hoiyin Cheung,Tong-Zhou Yu,Xin Yi,Yan-Jiao Wu,Qi Wang,Xue Gu,Miao Xu,Meihua Cai,Wen Wen,Xin-Ni Li,Ying-Xiao Liu,Ying Sun,Jijian Zheng,Tian-Le Xu,Yan Luo,Ma-Zhong Zhang,Wei-Guang Li
View all publications
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Product promise

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