Mouse Monoclonal GABA A Receptor gamma 2/GABRG2 antibody. Carrier free. Suitable for IHC-P and reacts with Rat, Mouse samples.
Images
![Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (AB307232), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-gamma-2-gabrg2-antibody-n452-69-bsa-and-azide-free-ab307232--immunohistochemistry-img406175.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (AB307232)")
![Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (AB307232), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-gamma-2-gabrg2-antibody-n452-69-bsa-and-azide-free-ab307232--immunohistochemistry-img406174.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (AB307232)")
![Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (AB307232), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-gamma-2-gabrg2-antibody-n452-69-bsa-and-azide-free-ab307232--immunohistochemistry-img406173.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (AB307232)")
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Gamma subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:14993607, PubMed:16412217, PubMed:23909897, PubMed:2538761, PubMed:25489750, PubMed:27864268, PubMed:29950725, PubMed:30602789). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed:29950725, PubMed:30602789). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed:14993607, PubMed:16412217, PubMed:2538761, PubMed:27864268, PubMed:29950725, PubMed:30602789). Gamma-2/GABRG2-containing GABAARs are found at both synaptic and extrasynaptic sites (By similarity). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (By similarity). GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation (PubMed:23909897, PubMed:25489750). Extrasynaptic gamma-2-containing receptors contribute to the tonic GABAergic inhibition (By similarity). GABAARs function also as histamine receptor where histamine binds at the interface of two neighboring beta subunits and potentiates GABA response in a gamma-2 subunit-controlled manner (By similarity).
Alternative names
Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, GABAAR subunit gamma-2, GABRG2
Recommended products
Mouse Monoclonal GABA A Receptor gamma 2/GABRG2 antibody. Carrier free. Suitable for IHC-P and reacts with Rat, Mouse samples.
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
N452/69
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (ab307232), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-gamma-2-gabrg2-antibody-n452-69-bsa-and-azide-free-ab307232--immunohistochemistry-img406175.jpg "Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (ab307232)")
Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (ab307232)
This data was developed using Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] ab307231, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling GABA A Receptor gamma 2/GABRG2 with Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] ab307231 at 1/500 dilution (1.654 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum is observed.
The section was incubated with Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] ab307231 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (ab307232), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-gamma-2-gabrg2-antibody-n452-69-bsa-and-azide-free-ab307232--immunohistochemistry-img406174.jpg "Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (ab307232)")
Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (ab307232)
This data was developed using Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] ab307231, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling GABA A Receptor gamma 2/GABRG2 with Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] ab307231 at 1/500 dilution (1.654 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse kidney.
The section was incubated with Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] ab307231 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (ab307232), expandable thumbnail](https://content.abcam.com/products/images/gaba-a-receptor-gamma-2-gabrg2-antibody-n452-69-bsa-and-azide-free-ab307232--immunohistochemistry-img406173.jpg "Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (ab307232)")
Immunohistochemistry - Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] - BSA and Azide free (ab307232)
This data was developed using Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] ab307231, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling GABA A Receptor gamma 2/GABRG2 with Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] ab307231 at 1/500 dilution (1.654 ug/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum is observed.
The section was incubated with Anti-GABA A Receptor gamma 2/GABRG2 antibody [N452/69] ab307231 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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