AB86186

Anti-GABA antibody [5A9]

Name: Anti-GABA antibody [5A9] (ab86186) | Abcam
Brand: Abcam Limited
SKU: AB86186
Price: 345 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(17 Publications)

Anti-GABA antibody [5A9] (ab86186) is a mouse monoclonal antibody detecting GABA in IHC-P, ICC/IF.

- Over 10 publications
- Trusted since 2009

View Alternative Names

4 aminobutanoic acid, Gamma amino butyric acid

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (AB86186)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (AB86186)

ab86186 staining GABA in the Brains of 2-5 days old moths (Manduca) by ICC/IF (Immunocytochemistry/immunofluorescence). The head capsules were fixed with 4% paraformaldehyde, washed in PBS and embedded in Low melting point Agarose and section to 75 μm thickness.Section were blocked with 2% BSA(IgG-free) . Samples were incubated with primary antibody (1/500 in PBS).

Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). Scale bar = 100 μm.

Image from Dacks AM et al., PLoS One. 2013;8(7):e69422. Fig 5-F.; doi: 10.1371/journal.pone.0069422.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (AB86186)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (AB86186)

Immunohistochemical analysis of human brain tissue labeling GABA with ab86186 at 1 : 20 dilution. Sample was incubated with primary antibody for 60 minutes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5A9

Isotype

IgG1

Carrier free

Applications

IHC-P, ICC/IF, IHC-FoFr

applications

Specificity

ab86186 is highly specific to GABA and does not react with other amino acid BSA conjugates with the exception of weak reaction against beta alanine BSA conjugate at the highest concentrations used.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-GABA antibody [5A9] (ab86186) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF). Trusted by the scientific community
Anti-GABA [5A9] (ab86186) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information GABA

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:295 PubMed40931022

2025

Astrocytic monoamine oxidase B (MAOB)-gamma-aminobutyric acid (GABA) axis as a molecular brake on repair following spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Yeong Lee,Jung Moo Lee,Hye-Lan Lee,Jiyeon Park,Heeyoung An,Eun Kyung Park,Sae Yeon Hwang,Sol Lip Yoon,Gwang Yong Hwang,Keung Nyun Kim,Min-Ho Nam,Seung Eun Lee,Hyunji Kang,Joungha Won,Bo Ko Jang,Elijah Hwejin Lee,SunYeong Choi,Mingu Gordon Park,Sang Wook Kim,Ki Duk Park,SeungHwan Lee,C Justin Lee,Yoon Ha

BMC biology 23:86 PubMed40140835

2025

Ectopic expression of Slc1a2 in the prefrontal cortex of sleep-deprived male mice counteracts the glutamate/GABA-glutamine dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Fengying Zhang,Yao Li,Li Jiang,Yingbin Wang,Yonghong Tang,Bo Ouyang,Guojun Song,Xuan Li,Xiajie Quan,Min Li,Hongying Wang,Zhao Pan,Keyan Chen,Ping Zhang

Journal of translational medicine 22:1158 PubMed39736794

2024

Taltirelin induces TH expression by regulating TRHR and RARα in medium spiny neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Kedong Zhu,Lanxia Meng,Jiaying Luo,Tingting Wen,Liang Dan,Zhihao Wang,Xuebing Cao,Zhaohui Zhang,Guiqin Chen

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39406519

2024

The Excessive Tonic Inhibition of the Peri-infarct Cortex Depresses Low Gamma Rhythm Power During Poststroke Recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Alasoadura,Juliette Leclerc,Mahmoud Hazime,Jérôme Leprince,David Vaudry,Julien Chuquet

Cell research 34:556-571 PubMed38849501

2024

A GABAergic system in atrioventricular node pacemaker cells controls electrical conduction between the atria and ventricles.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Liang,Liping Zhou,Huixing Zhou,Fulei Zhang,Guojian Fang,Junwei Leng,Yahan Wu,Yuemei Zhang,Anqi Yang,Yi Liu,Yi-Han Chen

Translational psychiatry 13:246 PubMed37414777

2023

Early maturation and hyperexcitability is a shared phenotype of cortical neurons derived from different ASD-associated mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Yara Hussein,Utkarsh Tripathi,Ashwani Choudhary,Ritu Nayak,David Peles,Idan Rosh,Tatiana Rabinski,Jose Djamus,Gad David Vatine,Ronen Spiegel,Tali Garin-Shkolnik,Shani Stern

Oxidative medicine and cellular longevity 2022:8469756 PubMed35663195

2022

Neonatal Oxidative Stress Impairs Cortical Synapse Formation and GABA Homeostasis in Parvalbumin-Expressing Interneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Till Scheuer,Stefanie Endesfelder,Elena Auf dem Brinke,Christoph Bührer,Thomas Schmitz

Frontiers in neuroscience 15:805070 PubMed34938159

2021

Effects of Nrf1 in Hypothalamic Paraventricular Nucleus on Regulating the Blood Pressure During Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jing Yu,Tong Xiao,Xiao-Jing Liu,Ying Li,Jie Qi,Nianping Zhang,Li-Yan Fu,Kai-Li Liu,Yanjun Li,Yu-Ming Kang

Journal of neurochemistry 158:373-390 PubMed33950542

2021

Activation of spinal PDGFRβ in microglia promotes neuronal autophagy via p38 MAPK pathway in morphine-tolerant rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqian Jia,Anqi Zhang,Zheng Li,Xiaoling Peng,Xuebi Tian,Feng Gao

EMBO molecular medicine 13:e12523 PubMed33501759

2021

Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Cleber A Trujillo,Jason W Adams,Priscilla D Negraes,Cassiano Carromeu,Leon Tejwani,Allan Acab,Ben Tsuda,Charles A Thomas,Neha Sodhi,Katherine M Fichter,Sarah Romero,Fabian Zanella,Terrence J Sejnowski,Henning Ulrich,Alysson R Muotri

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABA antibody [5A9]

Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (AB86186)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (AB86186)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (17)

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise