Anti-GABA antibody [5A9] (ab86186) is a mouse monoclonal antibody detecting GABA in IHC-P, ICC/IF.
- Over 10 publications
- Trusted since 2009
Images
![Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (AB86186), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-5a9-ab86186--immunocytochemistry-immunofluorescence-img73987.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (AB86186)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (AB86186), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-5a9-ab86186--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40875.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (AB86186)")
Publications
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- Oxidative medicine and cellular longevity 2022:84697562022Neonatal Oxidative Stress Impairs Cortical Synapse Formation and GABA Homeostasis in Parvalbumin-Expressing Interneurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTill Scheuer,Stefanie Endesfelder,Elena Auf dem Brinke,Christoph Bührer,Thomas SchmitzPubMed 35663195
- Frontiers in neuroscience 15:8050702021Effects of Nrf1 in Hypothalamic Paraventricular Nucleus on Regulating the Blood Pressure During Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Jing Yu,Tong Xiao,Xiao-Jing Liu,Ying Li,Jie Qi,Nianping Zhang,Li-Yan Fu,Kai-Li Liu,Yanjun Li,Yu-Ming KangPubMed 34938159
- Journal of neurochemistry 158:373-3902021Activation of spinal PDGFRβ in microglia promotes neuronal autophagy via p38 MAPK pathway in morphine-tolerant rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoqian Jia,Anqi Zhang,Zheng Li,Xiaoling Peng,Xuebi Tian,Feng GaoPubMed 33950542
- EMBO molecular medicine 13:e125232021Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCleber A Trujillo,Jason W Adams,Priscilla D Negraes,Cassiano Carromeu,Leon Tejwani,Allan Acab,Ben Tsuda,Charles A Thomas,Neha Sodhi,Katherine M Fichter,Sarah Romero,Fabian Zanella,Terrence J Sejnowski,Henning Ulrich,Alysson R MuotriPubMed 33501759
- Nature communications 11:32452020Dynamic perceptual feature selectivity in primary somatosensory cortex upon reversal learning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRonan Chéreau et. al.PubMed 32591523
- Cell stem cell 25:558-569.e72019Complex Oscillatory Waves Emerging from Cortical Organoids Model Early Human Brain Network Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCleber A Trujillo et. al.PubMed 31474560
- International journal of stem cells 12:315-3302019A Novel Immunomodulatory Mechanism Dependent on Acetylcholine Secreted by Human Bone Marrow-derived Mesenchymal Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTac-Ghee Yi,Yun-Kyoung Cho,Hyun-Joo Lee,Junghee Kim,Myung-Shin Jeon,Dong-Sik Ham,Woo Cheol Kim,Sun U SongPubMed 31242717
- Bio-protocol 9:e31882019Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesScott Bell,Nuwan C Hettige,Heika Silveira,Huashan Peng,Hanrong Wu,Malvin Jefri,Lilit Antonyan,Ying Zhang,Xin Zhang,Carl ErnstPubMed 33654990
- Experimental neurobiology 27:112-1192018Aucubin Promotes Differentiation of Neural Precursor Cells into GABAergic Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiyeoun Song et. al.PubMed 29731677
- Neural plasticity 2013:5306512013Cross-modal plasticity results in increased inhibition in primary auditory cortical areas.Applications:IHCReactive species:Unspecified reactive speciesYu-Ting Mao,Sarah L PallasPubMed 24288625
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Chemical / Small Molecule corresponding to GABA.
Reactivity data
Select an application| IHC-FoFr | IHC-P | ICC/IF | |
|---|---|---|---|
| Chemical | Expected | Tested | Tested |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Alternative names
4 aminobutanoic acid, Gamma amino butyric acid
Recommended products
Anti-GABA antibody [5A9] (ab86186) is a mouse monoclonal antibody detecting GABA in IHC-P, ICC/IF.
- Over 10 publications
- Trusted since 2009
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Chemical / Small Molecule corresponding to GABA.
- Clone number
- 5A9
- Purification technique
- Affinity purification Protein G
- Specificity
ab86186 is highly specific to GABA and does not react with other amino acid BSA conjugates with the exception of weak reaction against beta alanine BSA conjugate at the highest concentrations used.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-GABA antibody [5A9] (ab86186) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF).
Trusted by the scientific community
Anti-GABA [5A9] (ab86186) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (ab86186), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-5a9-ab86186--immunocytochemistry-immunofluorescence-img73987.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (ab86186)")
Image from Dacks AM et al., PLoS One. 2013;8(7):e69422. Fig 5-F.; doi: 10.1371/journal.pone.0069422.Immunocytochemistry/ Immunofluorescence - Anti-GABA antibody [5A9] (ab86186)
ab86186 staining GABA in the Brains of 2-5 days old moths (Manduca) by ICC/IF (Immunocytochemistry/immunofluorescence). The head capsules were fixed with 4% paraformaldehyde, washed in PBS and embedded in Low melting point Agarose and section to 75 μm thickness.Section were blocked with 2% BSA(IgG-free) . Samples were incubated with primary antibody (1/500 in PBS).
Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). Scale bar = 100 μm.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (ab86186), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-5a9-ab86186--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40875.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (ab86186)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [5A9] (ab86186)
Immunohistochemical analysis of human brain tissue labeling GABA with ab86186 at 1:20 dilution. Sample was incubated with primary antibody for 60 minutes.
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