Anti-GABA antibody [EPR24433-9]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (AB308437)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling GABA with ab308437 at 1/500 dilution (1.036 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab308437 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (AB308437)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling GABA with ab308437 at 1/500 dilution (1.036 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on rat skeletal muscle. The section was incubated with ab308437 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (AB308437)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling GABA with ab308437 at 1/500 dilution (1.036 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse hippocampus. The section was incubated with ab308437 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (AB308437)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling GABA with ab308437 at 1/500 dilution (1.036 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on mouse skeletal muscle. The section was incubated with ab308437 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• I-ELISA

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Indirect ELISA - Anti-GABA antibody [EPR24433-9] (AB308437)

Indirect ELISA - Anti-GABA antibody [EPR24433-9] (ab308437) used at 0-1000 ng/ml. Secondary used is Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at a 1/2500 dilution. This antibody does not cross-react with L-Aspartic acid.

• WB

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Western blot - Anti-GABA antibody [EPR24433-9] (AB308437)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. This antibody does not cross-react with L-Aspartic acid. GABA has a molecular weight of 103Da, OVA 45kd, and BSA 67kd. In lane1, the band at approximately 45 kDa is the GABA cross-linked to OVA. In lane2, the band over 50 kDa is the GABA cross-linked to BSA.

All lanes:

Western blot - Anti-GABA antibody [EPR24433-9] (ab308437) at 1/1000 dilution

Lane 1:

GABA cross-linked to OVA at 10 ng

Lane 2:

GABA cross-linked to BSA at 10 ng

Lane 3:

L-Aspartic acid cross-linked to BSA at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa,67 kDa

false

Exposure time: 103s