Rabbit Recombinant Monoclonal GABA antibody. Suitable for WB, IHC-P, I-ELISA and reacts with Chemical samples.
Images
![Western blot - Anti-GABA antibody [EPR24433-9] (AB308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--western-blot-img408798.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GABA antibody [EPR24433-9] (AB308437)")
![Indirect ELISA - Anti-GABA antibody [EPR24433-9] (AB308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--indirect-elisa-img420812.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-GABA antibody [EPR24433-9] (AB308437)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (AB308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408800.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (AB308437)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (AB308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408801.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (AB308437)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (AB308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408802.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (AB308437)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | I-ELISA | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Chemical | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1000 ng/mL | Notes Use at 62.5 ng/ml |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Alternative names
4 aminobutanoic acid, Gamma amino butyric acid
Recommended products
Rabbit Recombinant Monoclonal GABA antibody. Suitable for WB, IHC-P, I-ELISA and reacts with Chemical samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24433-9
- Purification technique
- Affinity purification Protein A
- Specificity
This RabMAb works well in mouse and rat brain tissues but not human. However, undetermined positive signals are found in human heart, liver, and testis for IHC.
WB application is recommended for testing GABA cross-linking proteins. We do not guarantee detection of endogenous GABA in WB.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-GABA antibody [EPR24433-9] (ab308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--western-blot-img408798.jpg "Western blot - Anti-GABA antibody [EPR24433-9] (ab308437)")
Western blot - Anti-GABA antibody [EPR24433-9] (ab308437)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with L-Aspartic acid.
GABA has a molecular weight of 103Da, OVA 45kd, and BSA 67kd.
In lane1, the band at approximately 45 kDa is the GABA cross-linked to OVA.
In lane2, the band over 50 kDa is the GABA cross-linked to BSA.All lanes: Western blot - Anti-GABA antibody [EPR24433-9] (ab308437) at 1/1000 dilution
Lane 1: GABA cross-linked to OVA at 10 ng
Lane 2: GABA cross-linked to BSA at 10 ng
Lane 3: L-Aspartic acid cross-linked to BSA at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 45 kDa, 67 kDa
Exposure time: 103s
![Indirect ELISA - Anti-GABA antibody [EPR24433-9] (ab308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--indirect-elisa-img420812.jpg "Indirect ELISA - Anti-GABA antibody [EPR24433-9] (ab308437)")
Indirect ELISA - Anti-GABA antibody [EPR24433-9] (ab308437)
Indirect ELISA - Anti-GABA antibody [EPR24433-9] (ab308437) used at 0-1000 ng/ml.
Secondary used is Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at a 1/2500 dilution.
This antibody does not cross-react with L-Aspartic acid.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408800.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling GABA with ab308437 at 1/500 dilution (1.036 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on rat cerebrum.
The section was incubated with ab308437 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408801.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling GABA with ab308437 at 1/500 dilution (1.036 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: No staining on rat skeletal muscle.
The section was incubated with ab308437 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408802.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling GABA with ab308437 at 1/500 dilution (1.036 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: No staining on mouse skeletal muscle.
The section was incubated with ab308437 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408799.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling GABA with ab308437 at 1/500 dilution (1.036 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on mouse hippocampus.
The section was incubated with ab308437 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437), expandable thumbnail](https://content.abcam.com/products/images/gaba-antibody-epr24433-9-ab308437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408799.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA antibody [EPR24433-9] (ab308437)")
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