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Mouse Monoclonal GABA B Receptor 1 antibody. Suitable for Flow Cyt, WB, IHC-FoFr, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 68 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GABBR1 aa 50-200.


Images

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.4
Constituents: 91% Water, 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Potassium chloride, 0.2% Potassium phosphate monobasic

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human GABBR1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBS5
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-FoFrICC/IFIHC-Fr
Human
Tested
Tested
Expected
Tested
Expected
Mouse
Predicted
Predicted
Expected
Predicted
Expected
Rat
Predicted
Predicted
Expected
Predicted
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872316, PubMed:9872744, PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:18165688). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10906333, PubMed:10773016, PubMed:10075644, PubMed:9872744, PubMed:24305054). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Calcium is required for high affinity binding to GABA (By similarity). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9844003). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9844003, PubMed:9872316, PubMed:10075644, PubMed:9872744, PubMed:22660477). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable). Activated by (-)-baclofen, cgp27492 and blocked by phaclofen (PubMed:9844003, PubMed:9872316, PubMed:24305054).Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.

Alternative names

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Mouse Monoclonal GABA B Receptor 1 antibody. Suitable for Flow Cyt, WB, IHC-FoFr, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 68 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GABBR1 aa 50-200.

Alternative names

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human GABBR1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBS5
Clone number

2D7

Purity

Tissue culture supernatant

Light chain type

kappa

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 29th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody [2D7] (ab55051), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody [2D7] (ab55051)

    ICC/IF image of ab55051 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55051, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-GABA B Receptor 1 antibody [2D7] (ab55051), expandable thumbnail

    Flow Cytometry - Anti-GABA B Receptor 1 antibody [2D7] (ab55051)

    Overlay histogram showing SH-SY5Y cells stained with ab55051 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55051, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-GABA B Receptor 1 antibody [2D7] (ab55051), expandable thumbnail

    Western blot - Anti-GABA B Receptor 1 antibody [2D7] (ab55051)

    GABA B Receptor 1 antibody (ab55051) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-GABA B Receptor 1 antibody [2D7] (AB55051)

    Predicted band size: 108 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody [2D7] (ab55051), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody [2D7] (ab55051)

    ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with L-Glutamate (ab120049), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of L-Glutamate, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120049 (L-Glutamate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody [2D7] (ab55051), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody [2D7] (ab55051)

    ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with NMDA (ab120052), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of NMDA, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120052 (NMDA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    This image was generated using the ascites version of the product.

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Product protocols

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