Rabbit Recombinant Monoclonal GABA B Receptor 1 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Images
![Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--western-blot-img10538.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)")
![Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--western-blot-img12046.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116817.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24345.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101895.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (AB238130)")
Publications
Filter by
- Frontiers in public health 11:10871612023Combined effects of EMP and RF field on emotional behavior in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTongzhou Qin,Liyuan Liu,Xing Wang,Ling Guo,Jiajin Lin,Junze Du,Yizhe Xue,Panpan Lai,Yuntao Jing,Guirong DingPubMed 37006533
- Journal of cellular biochemistry 123:863-8772022Loss of cytoplasmic FMR1-interacting protein 2 (CYFIP2) induces browning in 3T3-L1 adipocytes via repression of GABA-BR and activation of mTORC1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSubramani Manigandan,Jong Won YunPubMed 35233844
- Aging cell 21:e135732022Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Shen,Xiaolei Wu,Zhan Zhang,Di Zhang,Sihua Yang,Da XingPubMed 35199454
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-Fr | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Expected | Tested |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Tested | Tested |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054, PubMed:36103875, PubMed:9872316, PubMed:9872744). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:18165688). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10075644, PubMed:10773016, PubMed:10906333, PubMed:24305054, PubMed:9872744). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Calcium is required for high affinity binding to GABA (By similarity). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9844003). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:10075644, PubMed:22660477, PubMed:9844003, PubMed:9872316, PubMed:9872744). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable). Activated by (-)-baclofen, cgp27492 and blocked by phaclofen (PubMed:24305054, PubMed:9844003, PubMed:9872316). Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
Alternative names
GPRC3A, GABBR1, Gamma-aminobutyric acid type B receptor subunit 1, GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1
Recommended products
Rabbit Recombinant Monoclonal GABA B Receptor 1 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22954-47
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--western-blot-img10538.jpg "Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)")
Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All the lysates were unboiled.
Skeletal muscle is a low-expression tissue.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26033241, 10996466).
Exposure time: Lanes 1-2: 75 seconds Lanes 3-4: 3 minutes.
All lanes: Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130) at 1/1000 dilution
Lane 1: Mouse cerebellum tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa, 95 kDa
![Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--western-blot-img12046.jpg "Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)")
Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All the lysates were unboiled.
Skeletal muscle is a low-expression tissue. T
he expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26033241, 10996466).
Exposure time: Lane 1 3 minutes Lanes 2-3: 50 seconds.
All lanes: Western blot - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg
Lane 2: Human cerebellum tissue lysate at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa, 95 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116817.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on human liver. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238130).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24345.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebellum. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238130).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101895.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebellum. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238130).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57768.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling GABA B Receptor 1 with ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebellum. The section was incubated with ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238130).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-ab238130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57768.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com