Rabbit Recombinant Monoclonal GABA B Receptor 1 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (AB264069), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-bsa-and-azide-free-ab264069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116817.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (AB264069)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (AB264069), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-bsa-and-azide-free-ab264069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24345.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (AB264069)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (AB264069), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-bsa-and-azide-free-ab264069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101895.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (AB264069)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (AB264069), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-bsa-and-azide-free-ab264069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57768.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (AB264069)")
Publications
Filter by
- PNAS nexus 2:pgad0732023Digital spatial profiling of human parathyroid tumors reveals cellular and molecular alterations linked to vitamin D deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia-Ling Tu,Wenhan Chang,Julie A Sosa,James KohPubMed 36992820
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-Fr | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Expected | Tested |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Tested | Tested |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054, PubMed:36103875, PubMed:9872316, PubMed:9872744). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:18165688). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10075644, PubMed:10773016, PubMed:10906333, PubMed:24305054, PubMed:9872744). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Calcium is required for high affinity binding to GABA (By similarity). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9844003). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:10075644, PubMed:22660477, PubMed:9844003, PubMed:9872316, PubMed:9872744). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable). Activated by (-)-baclofen, cgp27492 and blocked by phaclofen (PubMed:24305054, PubMed:9844003, PubMed:9872316). Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
Alternative names
GPRC3A, GABBR1, Gamma-aminobutyric acid type B receptor subunit 1, GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1
Recommended products
Rabbit Recombinant Monoclonal GABA B Receptor 1 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22954-47
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab264069 is the carrier-free version of Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-bsa-and-azide-free-ab264069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116817.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling GABA B Receptor 1 with Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on human liver. The section was incubated with Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-bsa-and-azide-free-ab264069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24345.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling GABA B Receptor 1 with Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebellum. The section was incubated with Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-bsa-and-azide-free-ab264069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101895.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebellum. The section was incubated with Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069), expandable thumbnail](https://content.abcam.com/products/images/gaba-b-receptor-1-antibody-epr22954-47-bsa-and-azide-free-ab264069--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57768.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 1 antibody [EPR22954-47] - BSA and Azide free (ab264069)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling GABA B Receptor 1 with Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebellum. The section was incubated with Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GABA B Receptor 1 antibody [EPR22954-47] ab238130).
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