AB181736

Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7]

Name: Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (ab181736) | Abcam
Brand: Abcam Limited
SKU: AB181736
Price: 495 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Mouse Monoclonal GABA B Receptor 2/GABBR2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant fragment - Human, Transfected cell lysate - Human, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GABBR2 aa 300-400.

View Alternative Names

GPR51, GPRC3B, GABBR2, Gamma-aminobutyric acid type B receptor subunit 2, GABA-B receptor 2, GABA-B-R2, GABA-BR2, GABABR2, Gb2, G-protein coupled receptor 51, HG20

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (AB181736)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (AB181736)

Immunofluorescent analysis of HeLa cells labeling GABA B Receptor 2/GABBR2 with ab181736 at 1/200 (green). Blue : DRAQ5 fluorescent DNA dye.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (AB181736)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (AB181736)

Immunohistochemical analysis of paraffin embedded Human cerebellum tissue labeling GABA B Receptor 2/GABBR2 with ab181736 at 1/200.

Supplier Data

Western blot - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (AB181736)

All lanes:

Western blot - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (ab181736) at 1/500 dilution

Lane 1:

non-transfected HEK293 cell lysate

Lane 2:

GABA B Receptor 2/GABBR2 (aa 319-483)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 106 kDa

false

Supplier Data

Western blot - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (AB181736)

All lanes:

Western blot - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (ab181736) at 1/500 dilution

All lanes:

GABA B Receptor 2/GABBR2 (aa 319-483) recombinant protein

Predicted band size: 106 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G7F7

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GABBR2 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

O75899

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

This product was changed from ascites to supernatant. Lot no's high than GR290583-1 are from Tissue Culture Supernatant.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed : 15617512, PubMed : 18165688, PubMed : 22660477, PubMed : 24305054, PubMed : 9872316, PubMed : 9872744). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed : 18165688). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed : 10075644, PubMed : 10773016, PubMed : 24305054). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed : 10075644, PubMed : 10773016, PubMed : 10906333, PubMed : 9872744). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed : 22660477, PubMed : 9872744). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed : 10075644, PubMed : 22660477, PubMed : 9872316, PubMed : 9872744). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable).

See full target information GABBR2

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 15:1015906 PubMed36311027

2022

Protein phosphatase 2A regulation of GABA receptors normalizes ischemia-induced aberrant receptor trafficking and provides neuroprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Hleihil,Karthik Balakrishnan,Dietmar Benke

Frontiers in molecular neuroscience 14:726133 PubMed34539344

2021

Sustained Baclofen-Induced Activation of GABA Receptors After Cerebral Ischemia Restores Receptor Expression and Function and Limits Progressing Loss of Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Hleihil,Markus Vaas,Musadiq A Bhat,Karthik Balakrishnan,Dietmar Benke

The Journal of physiology 596:4299-4322 PubMed29917243

2018

Sex-specific activation of SK current by isoproterenol facilitates action potential triangulation and arrhythmogenesis in rabbit ventricles.

Applications

Unspecified application

Species

Unspecified reactive species

Mu Chen,Dechun Yin,Shuai Guo,Dong-Zhu Xu,Zhuo Wang,Zhenhui Chen,Michael Rubart-von der Lohe,Shien-Fong Lin,Thomas H Everett Iv,James N Weiss,Peng-Sheng Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7]

Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (AB181736)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (AB181736)

Western blot - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (AB181736)

Western blot - Anti-GABA B Receptor 2/GABBR2 antibody [5G7F7] (AB181736)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise