Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] (AB75838)

ab75838, at a 1/100 dilution, staining GABA B Receptor 2/GABBR2 in paraffin embedded human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] (AB75838)

Overlay histogram showing SH-SY5Y cells stained with ab75838 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75838, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] (AB75838)

ab75838 staining GABA B Receptor 2/GABBR2 in Rat Postnatal (P15) Cerebellum by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a 10mM citrate buffer, pH6. Samples were incubated with primary antibody (1/400 in PBS with 2% donkey serum) for 16 hours at 25°C. An Alexa Fluor® 488 conjugated Donkey anti-rabbit, polyclonal was used as the secondary antibody.

IHC and confocal microscopy for GABA B Receptor 2/GABBR2 (GABAB2) in the postnatal rat cerebellum. (A, C, E, G) Negative control by omission of the primary antibody at 10, 20, 40 and 60X. (B-B'', D-D'', F-F'', H-H'') Immunolabeling for GABAB2 (green) and/or the calcium-binding protein calbindin (red) at 10, 20, 40 and 60X. (I-K) Zooms of the insets shown in F-F''. Specific signal for GABAB2 is mainly observed in the molecular layer (ML) and internal granular layer (IGL) of the cerebellar grey matter. Purkinje cells (PkL) are enriched in GABAB2 especially at the surface of the dendritic trees (white arrowheads) oriented towards the ML. Golgi II cells (yellow arrowhead) located in the IGL are also positive for GABAB2. EGL : external granular layer. n : calbindin-positive nucleus of a Purkinje cell. Orange arrowhead : GABAB2-immunoreactive granular cell in the IGL. WM : white matter. An Olympus FV 1000 confocal microscope was used and images were processed with Adobe Photoshop CS4. Figure generated by Juan Vilchez Aruani and Estela Muñoz, IHEM, UNCuyo, CONICET, Mendoza, Argentina.

• WB

Lab

Western blot - Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] (AB75838)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time :

Lane 1 and 2 : 140 seconds
Lane 3 to 6 : 180 seconds

We recommend not to boil the samples after lysis to get desired WB results.

All lanes:

Western blot - Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] (ab75838) at 1/1000 dilution

Lane 1:

Human hippocampus lysates unboiled at 20 µg

Lane 2:

Human hippocampus lysates boiled at 20 µg

Lane 3:

Mouse brain lysates unboiled at 20 µg

Lane 4:

Mouse brain lysates boiled at 20 µg

Lane 5:

Rat brain lysates unboiled at 20 µg

Lane 6:

Rat brain lysates boiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 106 kDa

Observed band size: 106 kDa

false

• WB

Unknown

Western blot - Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] (AB75838)

All lanes:

Western blot - Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] (ab75838) at 1/500 dilution

All lanes:

human cerebellum lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 106 kDa

Observed band size: 106 kDa

false