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AB284713

Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y] - BSA and Azide free

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Rabbit Monoclonal GABA B Receptor 2/GABBR2 phospho S893 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

GPR51, GPRC3B, GABBR2, Gamma-aminobutyric acid type B receptor subunit 2, GABA-B receptor 2, GABA-B-R2, GABA-BR2, GABABR2, Gb2, G-protein coupled receptor 51, HG20

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y] - BSA and Azide free (AB284713)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y] - BSA and Azide free (AB284713)

This data was developed using ab68426, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of phosphorylated GABA B Receptor 2/GABBR2 expression on paraffin-embedded Human brain tissue using ab68426 at 1/100 dilution. Heat mediated antigen retrieval was performed, and an HRP-conjugated polymerized secondary antibody was used.

Western blot - Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y] - BSA and Azide free (AB284713)
  • WB

Unknown

Western blot - Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y] - BSA and Azide free (AB284713)

This data was developed using ab68426, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y] (<a href='/en-us/products/primary-antibodies/gaba-b-receptor-2-gabbr2-phospho-s893-antibody-ep2318y-ab68426'>ab68426</a>) at 1/1000 dilution

Lane 1:

Human brain lysate - untreated at 10 µg

Lane 2:

Human brain lysate - treated with AP at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

Predicted band size: 106 kDa

Observed band size: 106 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2318Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

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Product details

ab284713 is the carrier-free version of ab68426

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed : 15617512, PubMed : 18165688, PubMed : 22660477, PubMed : 24305054, PubMed : 9872316, PubMed : 9872744). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed : 18165688). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed : 10075644, PubMed : 10773016, PubMed : 24305054). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed : 10075644, PubMed : 10773016, PubMed : 10906333, PubMed : 9872744). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed : 22660477, PubMed : 9872744). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed : 10075644, PubMed : 22660477, PubMed : 9872316, PubMed : 9872744). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable).
See full target information GABBR2 pS893
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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