Anti-GABA Transporter 1 / GAT 1 antibody

• WB

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Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (AB426)

GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/mL

Lane 1:

Rat Hippocampus Tissue Lysate at 10 µg

Lane 2:

Spinal Cord (Rat) Tissue Lysate at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

Predicted band size: 67 kDa

Observed band size: 74 kDa

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Exposure time: 30s

• WB

CiteAb

Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (AB426)

GABA Transporter 1 / GAT 1 western blot using anti-GABA Transporter 1 / GAT 1 antibody ab426. Publication image and figure legend from Dadsetan, S., Balzano, T., et al., 2016, J Neuroinflammation, PubMed 27623772.

ab426 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab426 please see the product overview.

Altered membrane expression of GABA transporters in the cerebellum of PCS rats. Membrane expression of GAT3 (a) and GAT1 (b) was analyzed using the BS3 crosslinker procedure as described in the "Methods" section. Samples, incubated in the absence or presence of BS3, were subjected to western blot using antibodies against each transporter. Representative images are shown. Samples in the absence of BS3 represent the total amount of each protein. Samples in the presence of BS3 represent the non-membrane fraction. The intensities of the bands were quantified and membrane expression was calculated as the difference of intensity between samples without and with BS3. Values are expressed as percentage of control rats and are the mean ± SEM of 8 rats per group for GAT1; 6 sham rats treated with vehicle and 7 sham rats treated with infliximab; and 6 PCS rats treated with vehicle and 5 PCS rats treated with infliximab for GAT3. Values significantly different from control rats are indicated by asterisks and from PCS rats by a. * p < 0.05; a p < 0.05

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