AB177483

Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]

Name: Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) | Abcam
Brand: Abcam Limited
SKU: AB177483
Price: 340 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(7 Publications)

Rabbit Recombinant Monoclonal GABA Transporter 1 / GAT 1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

GABATR, GABT1, GAT1, SLC6A1, Sodium- and chloride-dependent GABA transporter 1, GAT-1, Solute carrier family 6 member 1

2 Images

Lab

Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (AB177483)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time :

Lane 1 to 2 : 20 seconds
Lane 3 to 6 : 8 seconds

We recommend not to boil the samples after lysis to get desired WB results.

All lanes:

Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/10000 dilution

Lane 1:

Human brain lysates unboiled at 20 µg

Lane 2:

Human brain lysates boiled at 20 µg

Lane 3:

Mouse brain unboiled at 20 µg

Lane 4:

Mouse brain boiled at 20 µg

Lane 5:

Rat brain unboiled at 20 µg

Lane 6:

Rat brain boiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

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Supplier Data

Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (AB177483)

All lanes:

Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/1000 dilution

All lanes:

Fetal brain lysate at 10 µg

Predicted band size: 67 kDa

false

Carrier free

Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12998

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates transport of gamma-aminobutyric acid (GABA) together with sodium and chloride and is responsible for the reuptake of GABA from the synapse (PubMed : 30132828). The translocation of GABA, however, may also occur in the reverse direction leading to the release of GABA (By similarity). The direction and magnitude of GABA transport is a consequence of the prevailing thermodynamic conditions, determined by membrane potential and the intracellular and extracellular concentrations of Na(+), Cl(-) and GABA (By similarity). Can also mediate sodium- and chloride-dependent transport of hypotaurine but to a much lower extent as compared to GABA (By similarity).

See full target information SLC6A1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409086 PubMed39804990

2025

TIA1-Mediated Stress Granules Promote the Neuroinflammation and Demyelination in Experimental Autoimmune Encephalomyelitis through Upregulating IL-31RA Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Hua,Lingting Jin,Zheyu Fang,Yiyun Weng,Yuan Zhang,Jingjing Zhang,Dewei Xie,Yang Tang,Siyu Guo,Yingying Huang,Yilin Dai,Jia Li,Zhihui Huang,Xu Zhang

Frontiers in neuroscience 17:1201294 PubMed37841690

2023

New insights of metabolite abnormalities in the thalamus of rats with iminodiproprionitrile-induced tic disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Jingru Yu,Xuan Yao,Xin Zhang,Juanjuan Hao

Advanced healthcare materials 11:e2201319 PubMed36165212

2022

Inflammatory Microenvironment-Responsive Nanomaterials Promote Spinal Cord Injury Repair by Targeting IRF5.

Applications

Unspecified application

Species

Unspecified reactive species

Dezun Ma,He Shen,Fangman Chen,Weiyuan Liu,Yannan Zhao,Zhifeng Xiao,Xianming Wu,Bing Chen,Junna Lu,Dan Shao,Jianwu Dai

Cell death & disease 13:759 PubMed36055989

2022

Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lingting Jin,Jingjing Zhang,Xin Hua,Xingxing Xu,Jia Li,Jiaojiao Wang,Mianxian Wang,Huitao Liu,Haoyu Qiu,Man Chen,Xu Zhang,Ying Wang,Zhihui Huang

NPJ Parkinson's disease 7:91 PubMed34625569

2021

Adjusting vascular permeability, leukocyte infiltration, and microglial cell activation to rescue dopaminergic neurons in rodent models of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Ying Cai,Xiao-Xiao Fu,Hong Jiang,Shu Han

Mucosal immunology 12:531-544 PubMed30523310

2018

Slc6a13 deficiency promotes Th17 responses during intestinal bacterial infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wenkai Ren,Yuexia Liao,Xueyan Ding,Ye Jiang,Jiameng Yan,Yaoyao Xia,Bie Tan,Zhijie Lin,Jielin Duan,Xinming Jia,Guan Yang,Jinping Deng,Congrui Zhu,Philip R Hardwidge,Junxia Li,Guoqiang Zhu,Yulong Yin

Cancer biology & therapy 19:282-291 PubMed29394133

2018

MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1.

Applications

Unspecified application

Species

Unspecified reactive species

Naibijiang Maolakuerban,Baihetiya Azhati,Hamulati Tusong,Asimujiang Abula,Anniwaer Yasheng,Ayiding Xireyazidan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]

Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (AB177483)

Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (AB177483)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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