Rabbit Recombinant Monoclonal GABA Transporter 1 / GAT 1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.
Images
![Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (AB177483), expandable thumbnail](https://content.abcam.com/products/images/gaba-transporter-1-gat-1-antibody-epr12998-ab177483--western-blot-img127240.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (AB177483)")
![Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (AB177483), expandable thumbnail](https://content.abcam.com/products/images/gaba-transporter-1-gat-1-antibody-epr12998-ab177483--western-blot-img41675.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (AB177483)")
Publications
Filter by
- Frontiers in neuroscience 17:12012942023New insights of metabolite abnormalities in the thalamus of rats with iminodiproprionitrile-induced tic disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingru Yu,Xuan Yao,Xin Zhang,Juanjuan HaoPubMed 37841690
- Advanced healthcare materials 11:e22013192022Inflammatory Microenvironment-Responsive Nanomaterials Promote Spinal Cord Injury Repair by Targeting IRF5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDezun Ma,He Shen,Fangman Chen,Weiyuan Liu,Yannan Zhao,Zhifeng Xiao,Xianming Wu,Bing Chen,Junna Lu,Dan Shao,Jianwu DaiPubMed 36165212
- Cell death & disease 13:7592022Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingting Jin,Jingjing Zhang,Xin Hua,Xingxing Xu,Jia Li,Jiaojiao Wang,Mianxian Wang,Huitao Liu,Haoyu Qiu,Man Chen,Xu Zhang,Ying Wang,Zhihui HuangPubMed 36055989
- NPJ Parkinson's disease 7:912021Adjusting vascular permeability, leukocyte infiltration, and microglial cell activation to rescue dopaminergic neurons in rodent models of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua-Ying Cai,Xiao-Xiao Fu,Hong Jiang,Shu HanPubMed 34625569
- Mucosal immunology 12:531-5442018Slc6a13 deficiency promotes Th17 responses during intestinal bacterial infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenkai Ren,Yuexia Liao,Xueyan Ding,Ye Jiang,Jiameng Yan,Yaoyao Xia,Bie Tan,Zhijie Lin,Jielin Duan,Xinming Jia,Guan Yang,Jinping Deng,Congrui Zhu,Philip R Hardwidge,Junxia Li,Guoqiang Zhu,Yulong YinPubMed 30523310
- Cancer biology & therapy 19:282-2912018MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaibijiang Maolakuerban et. al.PubMed 29394133
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Mediates transport of gamma-aminobutyric acid (GABA) together with sodium and chloride and is responsible for the reuptake of GABA from the synapse (PubMed:30132828). The translocation of GABA, however, may also occur in the reverse direction leading to the release of GABA (By similarity). The direction and magnitude of GABA transport is a consequence of the prevailing thermodynamic conditions, determined by membrane potential and the intracellular and extracellular concentrations of Na(+), Cl(-) and GABA (By similarity). Can also mediate sodium- and chloride-dependent transport of hypotaurine but to a much lower extent as compared to GABA (By similarity).
Alternative names
GABATR, GABT1, GAT1, SLC6A1, Sodium- and chloride-dependent GABA transporter 1, GAT-1, Solute carrier family 6 member 1
Recommended products
Rabbit Recombinant Monoclonal GABA Transporter 1 / GAT 1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12998
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483), expandable thumbnail](https://content.abcam.com/products/images/gaba-transporter-1-gat-1-antibody-epr12998-ab177483--western-blot-img127240.jpg "Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)")
Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)
Blocking and dilution buffer: 5% NFDM/TBST.Exposure time:
Lane 1 to 2: 20 seconds
Lane 3 to 6: 8 secondsWe recommend not to boil the samples after lysis to get desired WB results.
All lanes: Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/10000 dilution
Lane 1: Human brain lysates unboiled at 20 µg
Lane 2: Human brain lysates boiled at 20 µg
Lane 3: Mouse brain unboiled at 20 µg
Lane 4: Mouse brain boiled at 20 µg
Lane 5: Rat brain unboiled at 20 µg
Lane 6: Rat brain boiled at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
![Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483), expandable thumbnail](https://content.abcam.com/products/images/gaba-transporter-1-gat-1-antibody-epr12998-ab177483--western-blot-img41675.jpg "Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)")
Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)
All lanes: Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/1000 dilution
All lanes: Fetal brain lysate at 10 µg
Predicted band size: 67 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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