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AB259971

Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • Advanced Validation
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(1 Publication)

Rabbit Recombinant Monoclonal GABA Transporter 1 / GAT 1 antibody. Suitable for ICC/IF, IHC-Fr, IHC-P, mIHC and reacts with Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

Gabt1, Gat-1, Gat1, Slc6a1, Sodium- and chloride-dependent GABA transporter 1, GAT-1, Solute carrier family 6 member 1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)

Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining on rat retina (PMID : 2387399).The section was incubated with ab259971 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 dilution (0.982 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum (PMID : 21382352). The section was incubated with ab259971 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum (PMID : 2387399). The section was incubated with ab259971 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/50 (9.82 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse retina is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)

Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse retina (PMID : 18975268).The section was incubated with ab259971 for 30 mins at room temperature .The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)

Immunofluorescence staining of GABA Transporter 1 / GAT 1 using ab259971 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

The cells were then incubated overnight at +4°C with ab259971 at 5 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

Immunocytochemistry/ Immunofluorescence - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)

Immunofluorescence staining of GABA Transporter 1 / GAT 1 using ab259971 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

The cells were then incubated overnight at +4°C with ab259971 at 1 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

Multiplex immunohistochemistry - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse retina tissue staining RBPMS with ab309094 at a 1/2000 dilution, ab140603 anti-CPX used at 1/2000 dilution and ab259971 anti-GAT1 used at a 1/500 dilution.

Panel A : merged staining of anti-RBPMS (green; Opal™520), anti-CPX (magenta; Opal™690) and anti-GAT1 (gray; Opal™570) on mouse retina.

Panel B : anti-RBPMS staining ganglion cell layer in mouse retina.

Panel C : ant-CPX staining outer nuclear layer and inner nuclear layer in mouse retina.

Panel D : ant-GAT1 staining inner plexiform layer in mouse retina.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab309094, ab140603 and ab259971 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (AB259971)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat retina tissue staining RBPMS with ab309094 at a 1/2000 dilution, ab140603 anti-CPX used at 1/2000 dilution and ab259971 anti-GAT1 used at a 1/500 dilution.

Panel A : merged staining of anti-RBPMS (green; Opal™520), anti-CPX (magenta; Opal™690) and anti-GAT1 (gray; Opal™570) on rat retina.

Panel B : anti-RBPMS staining ganglion cell layer in rat retina.

Panel C : ant-CPX staining outer nuclear layer and inner nuclear layer in rat retina.

Panel D : ant-GAT1 staining inner plexiform layer in rat retina.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab309094, ab140603 and ab259971 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Carrier free

    Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24202-20

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P, ICC/IF, IHC-Fr, mIHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p>Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/500", "mIHC-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p>Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/499", "mIHC-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates transport of gamma-aminobutyric acid (GABA) together with sodium and chloride and is responsible for the reuptake of GABA from the synapse (PubMed : 15234345, PubMed : 16150932, PubMed : 30270321, PubMed : 8420981). The translocation of GABA, however, may also occur in the reverse direction leading to the release of GABA (By similarity). The direction and magnitude of GABA transport is a consequence of the prevailing thermodynamic conditions, determined by membrane potential and the intracellular and extracellular concentrations of Na(+), Cl(-) and GABA (By similarity). Can also mediate sodium- and chloride-dependent transport of hypotaurine but to a much lower extent as compared to GABA (PubMed : 30270321).
See full target information Slc6a1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Drug design, development and therapy 19:2677-2691 PubMed40226128

2025

Huyang Yangkun Formula Enhances Ovarian Function and Delays Reproductive Aging by Influencing Hypothalamic GnRH/LH Pulse Release Through GAT-1/GABA/GABAAR2.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Yin Peng,Guangning Nie,Fangping Cheng,Yuling Zhou,Jian Liu,Hongyan Yang
View all publications
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Product promise

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