Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (AB277642)

This data was developed using ab259971, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) . Positive staining on rat retina (PMID : 2387399).The section was incubated with ab259971 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (AB277642)

This data was developed using ab259971, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 dilution (0.982 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on mouse cerebrum (PMID : 21382352). The section was incubated with ab259971 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (AB277642)

This data was developed using ab259971, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on rat cerebrum (PMID : 2387399). The section was incubated with ab259971 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (AB277642)

This data was developed using ab259971, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/50 (9.82 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse retina is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (AB277642)

This data was developed using ab259971, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on mouse retina (PMID : 18975268).The section was incubated with ab259971 for 30 mins at room temperature .The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (AB277642)

This data was developed using ab259971, the same antibody clone in a different buffer formulation.

Immunofluorescence staining of GABA Transporter 1 / GAT 1 using ab259971 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

The cells were then incubated overnight at +4°C with ab259971 at 5 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (AB277642)

This data was developed using ab259971, the same antibody clone in a different buffer formulation.

Immunofluorescence staining of GABA Transporter 1 / GAT 1 using ab259971 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

The cells were then incubated overnight at +4°C with ab259971 at 1 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (AB277642)

This data was developed using ab259971, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse retina tissue staining RBPMS with ab309094 at a 1/2000 dilution, ab140603 anti-CPX used at 1/2000 dilution and ab259971 anti-GAT1 used at a 1/500 dilution.

Panel A : merged staining of anti-RBPMS (green; Opal™520), anti-CPX (magenta; Opal™690) and anti-GAT1 (gray; Opal™570) on mouse retina.

Panel B : anti-RBPMS staining ganglion cell layer in mouse retina.

Panel C : ant-CPX staining outer nuclear layer and inner nuclear layer in mouse retina.

Panel D : ant-GAT1 staining inner plexiform layer in mouse retina.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab309094, ab140603 and ab259971 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (AB277642)

This data was developed using ab259971, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat retina tissue staining RBPMS with ab309094 at a 1/2000 dilution, ab140603 anti-CPX used at 1/2000 dilution and ab259971 anti-GAT1 used at a 1/500 dilution.

Panel A : merged staining of anti-RBPMS (green; Opal™520), anti-CPX (magenta; Opal™690) and anti-GAT1 (gray; Opal™570) on rat retina.

Panel B : anti-RBPMS staining ganglion cell layer in rat retina.

Panel C : ant-CPX staining outer nuclear layer and inner nuclear layer in rat retina.

Panel D : ant-GAT1 staining inner plexiform layer in rat retina.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab309094, ab140603 and ab259971 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.