Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364) is a rabbit monoclonal antibody that is used to detect GABARAP+GABARAPL1+GABARAPL2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 60 publications
Images
![Immunocytochemistry/ Immunofluorescence - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--immunocytochemistry-immunofluorescence-img30563.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108390.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364)")
![Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--flow-cytometry-intracellular-img13478.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364)")
![Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--western-blot-img160325.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364)")
![Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--western-blot-img62111.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364)")
Publications
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- Cell death & disease 14:5402023The deubiquitinase Leon/USP5 interacts with Atg1/ULK1 and antagonizes autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYueh-Ling Pai,Yuchieh Jay Lin,Wen-Hsin Peng,Li-Ting Huang,He-Yen Chou,Chien-Hsiang Wang,Cheng-Ting Chien,Guang-Chao ChenPubMed 37607937
- PLoS biology 21:e30022442023FBXO7/ntc and USP30 antagonistically set the ubiquitination threshold for basal mitophagy and provide a target for Pink1 phosphorylation in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlvaro Sanchez-Martinez,Aitor Martinez,Alexander J WhitworthPubMed 37535686
- Nature communications 14:30772023LC3-associated phagocytosis promotes glial degradation of axon debris after injury in Drosophila models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesÁron Szabó,Virág Vincze,Aishwarya Sanjay Chhatre,András Jipa,Sarolta Bognár,Katalin Eszter Varga,Poulami Banik,Adél Harmatos-Ürmösi,Lukas J Neukomm,Gábor JuhászPubMed 37248218
- Cell communication and signaling : CCS 21:1202023Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKlara Hanelova,Martina Raudenska,Monika Kratochvilova,Jiri Navratil,Tomas Vicar,Maria Bugajova,Jaromir Gumulec,Michal Masarik,Jan BalvanPubMed 37226246
- Nature communications 14:29322023Allnighter pseudokinase-mediated feedback links proteostasis and sleep in Drosophila.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShashank Shekhar,Andrew T Moehlman,Brenden Park,Michael Ewnetu,Charles Tracy,Iris Titos,Krzysztof Pawłowski,Vincent S Tagliabracci,Helmut KrämerPubMed 37217484
- Nature communications 14:28132023Small-sample learning reveals propionylation in determining global protein homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Shui,Chenwei Wang,Xuedi Zhang,Shanshan Ma,Qinyu Li,Wanshan Ning,Weizhi Zhang,Miaomiao Chen,Di Peng,Hui Hu,Zheng Fang,Anyuan Guo,Guanjun Gao,Mingliang Ye,Luoying Zhang,Yu XuePubMed 37198164
- Nature communications 14:21622023Next-generation large-scale binary protein interaction network for Drosophila melanogaster.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Wen Tang,Kerstin Spirohn,Yanhui Hu,Tong Hao,István A Kovács,Yue Gao,Richard Binari,Donghui Yang-Zhou,Kenneth H Wan,Joel S Bader,Dawit Balcha,Wenting Bian,Benjamin W Booth,Atina G Coté,Steffi de Rouck,Alice Desbuleux,Kah Yong Goh,Dae-Kyum Kim,Jennifer J Knapp,Wen Xing Lee,Irma Lemmens,Cathleen Li,Mian Li,Roujia Li,Hyobin Julianne Lim,Yifang Liu,Katja Luck,Dylan Markey,Carl Pollis,Sudharshan Rangarajan,Jonathan Rodiger,Sadie Schlabach,Yun Shen,Dayag Sheykhkarimli,Bridget TeeKing,Frederick P Roth,Jan Tavernier,Michael A Calderwood,David E Hill,Susan E Celniker,Marc Vidal,Norbert Perrimon,Stephanie E MohrPubMed 37061542
- mBio 14:e02919222023Mindin Activates Autophagy for Lipid Utilization and Facilitates White Spot Syndrome Virus Infection in Shrimp.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Gao,Ye-Jia Song,Hao Wang,Bao-Rui Zhao,Xian-Wei WangPubMed 36779788
- International journal of molecular sciences 24:2023The Activation of Reticulophagy by ER Stress through the ATF4-MAP1LC3A-CCPG1 Pathway in Ovarian Granulosa Cells Is Linked to Apoptosis and Necroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiduo Li,Yanan Jing,Xiaoya Qu,Jinyi Yang,Pengge Pan,Xinrui Liu,Hui Gao,Xiuying Pei,Cheng Zhang,Yanzhou YangPubMed 36769070
- Insects 14:2023Treatment of Rapamycin and Evaluation of an Autophagic Response in the Gut of (Sulč).Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunepyo Oh,Cecilia TamborindeguyPubMed 36835711
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Not recommended |
| Mouse | Tested | Expected | Expected | Expected | Not recommended |
| Rat | Tested | Expected | Expected | Expected | Not recommended |
| Silk worm | Predicted | Predicted | Predicted | Predicted | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Silk worm | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Mouse | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Silk worm | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20 | Notes - |
Species Rat | Dilution info 1/20 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Silk worm | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes Antigen retrieval is recommended. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Silk worm | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Silk worm | Dilution info - | Notes - |
Target data
Function
Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355). Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538). Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205). Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205). Involved in apoptosis (PubMed:15977068).
Additional Targets
GABARAPL1, GABARAPL2
Alternative names
FLC3B, HT004, GABARAP, Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, MM46
Recommended products
Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364) is a rabbit monoclonal antibody that is used to detect GABARAP+GABARAPL1+GABARAPL2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 60 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4805
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody will also recognize related targets GABARAPL1 and GABARAPL2:GABARAPL1: pBLAST 100% immunogen homology AND Abreview 38385 WB data GABARAPL2: pBLAST 93% homology (only 1aa gap of 14aa) AND Abreview 38385 WB data
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.
What is the molecular weight of GABARAP+GABARAPL1+GABARAPL2?
Anti-GABARAP+GABARAPL1+GABARAPL2 [EPR4805] (ab109364) specifically detects a band for GABARAP+GABARAPL1+GABARAPL2 (UniProt: O95166) at a molecular weight of 14kDa.
Recommended positive controls
Fetal kidney lysate, fetal brain lysate, SH-SY5Y cell lysate, HepG2 whole cell lysate (ab7900), human brain tissue, HeLa cells.
Trusted by the scientific community
Anti-GABARAP+GABARAPL1+GABARAPL2 [EPR4805] (ab109364) was first used in a scientific publication in 2011 and has been cited over 60 times in peer-reviewed journals.
Reviewed by scientists
Anti-GABARAP+GABARAPL1+GABARAPL2 [EPR4805] (ab109364) has over 10 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [EPR4805] also available for your convenience:
ab109364, HRP - HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] ab201813, Carrier free - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] - BSA and Azide free ab225535, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] ab319582, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] ab319688, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] ab319818, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] ab319959, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] ab320947
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--immunocytochemistry-immunofluorescence-img30563.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
Immunocytochemistry/ Immunofluorescence - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling GABARAP+GABARAPL1+GABARAPL2 with purified ab109364 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108390.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling GABARAP+GABARAPL1+GABARAPL2 with Purified ab109364 at 1:500 dilution (0.29 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
![Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--flow-cytometry-intracellular-img13478.jpg "Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GABARAP+GABARAPL1+GABARAPL2 with purified ab109364 at 1/20 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--western-blot-img160325.jpg "Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364) at 1/5000 dilution
All lanes: Rat kidney lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa
![Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--western-blot-img62111.jpg "Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364) at 1/5000 dilution
Lane 1: Mouse kidney lysates at 20 µg
Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa
![Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--western-blot-img103024.jpg "Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)
All lanes: Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364) at 1/1000 dilution
Lane 1: Fetal kidney lysate at 10 µg
Lane 2: SH-SY5Y cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: Fetal brain lysate at 10 µg
Predicted band size: 14 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136182.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)
Unpurified ab109364, at 1/500, staining GABARAP+GABARAPL1+GABARAPL2 in Human brain tissue by immunohistochemistry.
![Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--flow-cytometry-intracellular-img414369.jpg "Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)
Unpurified ab109364 staining GABARAP+GABARAPL1+GABARAPL2 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
![Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--western-blot-img103024.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136182.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
![Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364), expandable thumbnail](https://content.abcam.com/products/images/gabarapgabarapl1gabarapl2-antibody-epr4805-ab109364--flow-cytometry-intracellular-img414369.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)")
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