AB229558

Anti-GABARAPL1 antibody - N-terminal

Name: Anti-GABARAPL1 antibody - N-terminal (ab229558) | Abcam
Brand: Abcam Limited
SKU: AB229558
Price: 220 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GABARAPL1 antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GABARAPL1.

View Alternative Names

GEC1, GABARAPL1, Gamma-aminobutyric acid receptor-associated protein-like 1, Early estrogen-regulated protein, GABA(A) receptor-associated protein-like 1, Glandular epithelial cell protein 1, GEC-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-GABARAPL1 antibody - N-terminal (AB229558)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABARAPL1 antibody - N-terminal (AB229558)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GABARAPL1 (green) using ab229558 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain : Hoechst 33342 (blue) and phalloidin, a cytoskeleton marker (red)

Supplier Data

Western blot - Anti-GABARAPL1 antibody - N-terminal (AB229558)

15% SDS-PAGE

All lanes:

Western blot - Anti-GABARAPL1 antibody - N-terminal (ab229558) at 1/1000 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extracts at 60 µg

Lane 2:

SK-N-SH (Human neuroblastoma cell line) whole cell extracts at 60 µg

Lane 3:

Mouse brain tissue extracts at 15 µg

Lane 4:

Rat brain tissue extracts at 15 µg

Predicted band size: 14 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human GABARAPL1. The exact immunogen used to generate this antibody is proprietary information.

Q9H0R8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor (PubMed : 16431922). Involved in formation of autophagosomal vacuoles (PubMed : 20404487). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20404487). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538).

See full target information GABARAPL1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 136: PubMed37701987

2023

Loss of ATG4B and ATG4A results in two-stage cell cycle defects in pancreatic ductal adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Paalini Sathiyaseelan,Suganthi Chittaranjan,Steve E Kalloger,Jennifer Chan,Nancy E Go,Mario A Jardon,Cally J Ho,Theodore Hui,Jing Xu,Christine Chow,Dongxia Gao,Fraser D Johnson,William W Lockwood,Gregg B Morin,Daniel J Renouf,David F Schaeffer,Sharon M Gorski

RNA (New York, N.Y.) 29:1673-1690 PubMed37562960

2023

In vitro methylation of the U7 snRNP subunits Lsm11 and SmE by the PRMT5/MEP50/pICln methylosome.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Cui Yang,Anthony Desotell,Min-Han Lin,Andrew S Paige,Agata Malinowska,Yadong Sun,Wei Shen Aik,Michał Dadlez,Liang Tong,Zbigniew Dominski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABARAPL1 antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-GABARAPL1 antibody - N-terminal (AB229558)

Western blot - Anti-GABARAPL1 antibody - N-terminal (AB229558)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise