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Rabbit Recombinant Monoclonal GABARAPL1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

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Images

Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (AB191887), expandable thumbnail
  • Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (AB191887), expandable thumbnail
  • Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (AB191887), expandable thumbnail
  • Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (AB191887), expandable thumbnail
  • Immunoprecipitation - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (AB191887), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested
Recombinant full length protein - Human
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/40
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor (PubMed:16431922). Involved in formation of autophagosomal vacuoles (PubMed:20404487). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20404487). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538).

Additional Targets

GABARAPL3

Alternative names

GEC1, GABARAPL1, Gamma-aminobutyric acid receptor-associated protein-like 1, Early estrogen-regulated protein, GABA(A) receptor-associated protein-like 1, Glandular epithelial cell protein 1, GEC-1

Recommended products

Rabbit Recombinant Monoclonal GABARAPL1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18869
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887), expandable thumbnail

    Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    In house recombinant Human GABARAPL1 is a GST-tagged full length protein (aa1-117).

    All lanes: Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/5000 dilution

    All lanes: Recombinant human GABARAPL1 full length protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa

    Observed band size: 43 kDa

    Exposure time: 1s

  • Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887), expandable thumbnail

    Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All three recombinant Human full length proteins contain aa1-117 with an N-terminal His-Tag® and were made in-house.

    All lanes: Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/5000 dilution

    Lane 1: Recombinant Human GABARAPL2 full length protein at 0.01 µg

    Lane 2: Recombinant Human GABARAPL3 full length protein at 0.01 µg

    Lane 3: Recombinant Human GABARAP (O95166) full length protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa

    Observed band size: 17 kDa

    Exposure time: 1s

  • Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887), expandable thumbnail

    Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2,3 and 4: 30 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).

    All lanes: Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution

    Lane 1: Human fetal brain tissue lysate at 10 µg

    Lane 2: Human fetal liver tissue lysate at 10 µg

    Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 4: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 14 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887), expandable thumbnail

    Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).

    All lanes: Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Mouse kidney tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa

    Observed band size: 17 kDa

  • Immunoprecipitation - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887), expandable thumbnail

    Immunoprecipitation - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

    GABARAPL1 + GABARAPL3 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab191887 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab191887 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10ug (Input).

    Lane 2: ab191887 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab191887 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

    Observed band size: 17 kDa

  • Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887), expandable thumbnail

    Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).

    All lanes: Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution

    Lane 1: Rat brain lysate at 10 µg

    Lane 2: Rat kidney lysate at 10 µg

    Lane 3: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 4: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 5: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa

    Observed band size: 17 kDa

    Exposure time: 30s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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