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AB251099

Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free

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Rabbit Recombinant Monoclonal GABARAPL1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

View Alternative Names

GEC1, GABARAPL1, Gamma-aminobutyric acid receptor-associated protein-like 1, Early estrogen-regulated protein, GABA(A) receptor-associated protein-like 1, Glandular epithelial cell protein 1, GEC-1

6 Images
Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)
  • WB

Supplier Data

Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)

This data was developed using ab191887, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 15 seconds; Lanes 2-4 : 30 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 16650615).

All lanes:

Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (<a href='/en-us/products/primary-antibodies/gabarapl1-gabarapl3-antibody-epr18869-ab191887'>ab191887</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa

false

Immunoprecipitation - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)
  • IP

Supplier Data

Immunoprecipitation - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)

This data was developed using ab191887, the same antibody clone in a different buffer formulation.

GABARAPL1 + GABARAPL3 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab191887 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab191887 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input).

Lane 2 : ab191887 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab191887 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (<a href='/en-us/products/primary-antibodies/gabarapl1-gabarapl3-antibody-epr18869-ab191887'>ab191887</a>)

Observed band size: 17 kDa

false

Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)
  • WB

Supplier Data

Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)

This data was developed using ab191887, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 16650615).

All lanes:

Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (<a href='/en-us/products/primary-antibodies/gabarapl1-gabarapl3-antibody-epr18869-ab191887'>ab191887</a>) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Rat kidney lysate at 10 µg

Lane 3:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 5:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa

false

Exposure time: 30s

Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)
  • WB

Supplier Data

Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)

This data was developed using ab191887, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

In house recombinant Human GABARAPL1 is a GST-tagged full length protein (aa1-117).

All lanes:

Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (<a href='/en-us/products/primary-antibodies/gabarapl1-gabarapl3-antibody-epr18869-ab191887'>ab191887</a>) at 1/5000 dilution

All lanes:

Recombinant human GABARAPL1 full length protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 14 kDa

Observed band size: 43 kDa

false

Exposure time: 1s

Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)
  • WB

Supplier Data

Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)

This data was developed using ab191887, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All three recombinant Human full length proteins contain aa1-117 with an N-terminal His-Tag® and were made in-house.

All lanes:

Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (<a href='/en-us/products/primary-antibodies/gabarapl1-gabarapl3-antibody-epr18869-ab191887'>ab191887</a>) at 1/5000 dilution

Lane 1:

Recombinant Human GABARAPL2 full length protein at 0.01 µg

Lane 2:

Recombinant Human GABARAPL3 full length protein at 0.01 µg

Lane 3:

Recombinant Human GABARAP (O95166) full length protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa

false

Exposure time: 1s

Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)
  • WB

Supplier Data

Western blot - Anti-GABARAPL1+GABARAPL3 antibody [EPR18869] - BSA and Azide free (AB251099)

This data was developed using ab191887, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 5 seconds; Lane 2 : 15 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 16650615).

All lanes:

Western blot - Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (<a href='/en-us/products/primary-antibodies/gabarapl1-gabarapl3-antibody-epr18869-ab191887'>ab191887</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa

false

  • Unconjugated

    Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18869

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251099 is the carrier-free version of ab191887.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor (PubMed : 16431922). Involved in formation of autophagosomal vacuoles (PubMed : 20404487). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20404487). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538).
See full target information GABARAPL1

Additional targets

GABARAPL3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com