AB137511

Anti-GABARAPL2/GATE-16 antibody

Name: Anti-GABARAPL2/GATE-16 antibody (ab137511) | Abcam
Brand: Abcam Limited
SKU: AB137511
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GABARAPL2/GATE-16 antibody. Suitable for IHC-P, WB, IHC - Wmt and reacts with Human, Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GABARAPL2 aa 1 to C-terminus.

View Alternative Names

FLC3A, GEF2, GABARAPL2, Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, MAP1 light chain 3-related protein, GEF-2, GATE-16

3 Images

Supplier Data

Western blot - Anti-GABARAPL2/GATE-16 antibody (AB137511)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-GABARAPL2/GATE-16 antibody (ab137511) at 1/1500 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Predicted band size: 14 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAPL2/GATE-16 antibody (AB137511)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAPL2/GATE-16 antibody (AB137511)

Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labelling GABARAPL2/GATE-16 with ab137511 at 1/100 dilution.

IHC - Wholemount - Anti-GABARAPL2/GATE-16 antibody (AB137511)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-GABARAPL2/GATE-16 antibody (AB137511)

GABARAPL2 antibody detects Gabarapl2 protein on zebrafish by whole mount immunohistochemical analysis.
ab137511 was used at a 1/50 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Zebrafish

Applications

IHC-P, IHC - Wmt, WB

applications

Immunogen

Recombinant Fragment Protein within Human GABARAPL2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P60520

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-like modifier involved in intra-Golgi traffic (By similarity). Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation (By similarity). It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy (PubMed : 20418806, PubMed : 23209295). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed : 20418806, PubMed : 23209295). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20418806, PubMed : 23209295).

See full target information GABARAPL2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 8: PubMed31309927

2019

Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Kh Stavoe,Pallavi P Gopal,Andrea Gubas,Sharon A Tooze,Erika Lf Holzbaur

PloS one 9:e108049 PubMed25229239

2014

EGF up-regulates miR-31 through the C/EBPβ signal cascade in oral carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Cheng Lu,Shou-Yen Kao,Cheng-Chieh Yang,Hsi-Feng Tu,Cheng-Hsien Wu,Kuo-Wei Chang,Shu-Chun Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABARAPL2/GATE-16 antibody

Western blot - Anti-GABARAPL2/GATE-16 antibody (AB137511)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAPL2/GATE-16 antibody (AB137511)

IHC - Wholemount - Anti-GABARAPL2/GATE-16 antibody (AB137511)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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