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AB325858

Anti-GABBR1 antibody [GT83] - BSA and Azide free

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Mouse Recombinant Monoclonal GABA B Receptor 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human GABBR1.

View Alternative Names

Gamma-aminobutyric acid type B receptor subunit 1, GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1, Gabbr1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)

ab325858 detects GABA B Receptor 1 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat colon. GABA B Receptor 1 stained by ab325858 diluted at 1 : 100. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)

ab325858 detects GABA B Receptor 1 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat tissues. GABA B Receptor 1 stained by ab325858 diluted at 1 : 100. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)

ab325858 detects GABA B Receptor 1 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. GABA B Receptor 1 stained by ab325858 diluted at 1 : 100. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)

ab325858 detects GABA B Receptor 1 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse cerebellum. GABA B Receptor 1 stained by ab325858 diluted at 1 : 100. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)

ab325858 detects GABA B Receptor 1 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat tissues. GABA B Receptor 1 stained by diluted at 1 : 100. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Western blot - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)
  • WB

Supplier Data

Western blot - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)

Unboiled various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ab325858 diluted at 1 : 1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GABBR1 antibody [GT83] - BSA and Azide free (ab325858) at 1/1000 dilution

Lane 1:

Unboiled mouse brain tissue extracts at 50 µg

Lane 2:

Unboiled mouse kidney tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)
  • WB

Supplier Data

Western blot - Anti-GABBR1 antibody [GT83] - BSA and Azide free (AB325858)

Unboiled various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ab325858 diluted at 1 : 1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-GABBR1 antibody [GT83] - BSA and Azide free (ab325858) at 1/1000 dilution

Lane 1:

Unboiled mouse brain tissue extracts at 50 µg

Lane 2:

Unboiled rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT83

Isotype

IgG2b

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GABBR1.

Q9UBS5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed : 9872315, PubMed : 9872317, PubMed : 9872744). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed : 10658574, PubMed : 9872317). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed : 10075644, PubMed : 10924501, PubMed : 9872315, PubMed : 9872744). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed : 10457184, PubMed : 10692480, PubMed : 10924501, PubMed : 9069281, PubMed : 9872315, PubMed : 9872744). Calcium is required for high affinity binding to GABA (PubMed : 10692480). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed : 9872744). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed : 10692480, PubMed : 10924501, PubMed : 9872744). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity).
See full target information Gabbr1
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Product promise

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