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AB224325

Anti-GABPA antibody

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(5 Publications)

Rabbit Polyclonal GABPA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GABPA aa 1-150.

View Alternative Names

E4TF1A, GABPA, GA-binding protein alpha chain, GABP subunit alpha, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GABPA antibody (AB224325)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABPA antibody (AB224325)

PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for GABPA (green) using ab224325 at 4 μg/ml in ICC/IF.

Western blot - Anti-GABPA antibody (AB224325)
  • WB

Supplier Data

Western blot - Anti-GABPA antibody (AB224325)

All lanes:

Western blot - Anti-GABPA antibody (ab224325) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Lane 3:

Human liver tissue lysate

Lane 4:

Human tonsil tissue lysate

Predicted band size: 51 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABPA antibody (AB224325)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABPA antibody (AB224325)

Paraffin-embedded human lymph node tissue stained for GABPA using ab224325 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GABPA aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q06546

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor capable of interacting with purine rich repeats (GA repeats). Positively regulates transcription of transcriptional repressor RHIT/ZNF205 (PubMed : 22306510).. (Microbial infection) Necessary for the expression of the Adenovirus E4 gene.
See full target information GABPA
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111487 PubMed39759007

2025

miR-450b-5p promotes development of endometriosis by inhibiting the GABPA/HOXD10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Huang,Yidan Wang,Ruiyun Li,Yongmei Liu,Yuan Yang

Scientific reports 14:26521 PubMed39489850

2024

GABPA inhibits tumorigenesis in clear cell renal cell carcinoma by regulating ferroptosis through ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqian Wu,Zonglong Wu,Mengfei Yao,Li Liu,Yimeng Song,Lulin Ma,Cheng Liu

Ageing research reviews 98:102353 PubMed38815934

2024

Unraveling the NRF2 confusion: Distinguishing nuclear respiratory factor 2 from nuclear erythroid factor 2.

Applications

Unspecified application

Species

Unspecified reactive species

Mathew George,Arubala P Reddy,P Hemachandra Reddy,Sudhir Kshirsagar

Journal of cell science 135: PubMed35099001

2022

Drp1 regulates transcription of ribosomal protein genes in embryonic hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Qiancong Zhao,Shun Yan,Jin Lu,Danitra J Parker,Huiying Wu,Qianchuang Sun,David K Crossman,Shanrun Liu,Qin Wang,Hiromi Sesaki,Kasturi Mitra,Kexiang Liu,Kai Jiao

Molecular biology of the cell 33:br6 PubMed35044843

2022

Ca entry through mechanotransduction channels localizes BAIAP2L2 to stereocilia tips.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Halford,Michael Bateschell,Peter G Barr-Gillespie
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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