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AB88746

Anti-GABPB2/Nuclear respiratory factor 2 antibody

4

(4 Reviews)

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(3 Publications )

Mouse Polyclonal Nuclear respiratory factor 2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GABPB1.
5 Images
Immunocytochemistry/ Immunofluorescence - Anti-GABPB2/Nuclear respiratory factor 2 antibody (AB88746)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GABPB2/Nuclear respiratory factor 2 antibody (AB88746)

ab88746 at 10 μg/ml staining Nuclear respiratory factor 2 in PFA-fixed, permeabilised HeLa cells by immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABPB2/Nuclear respiratory factor 2 antibody (AB88746)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABPB2/Nuclear respiratory factor 2 antibody (AB88746)

ab88746 at 3 μg/ml staining Nuclear respiratory factor 2 in formalin-fixed, paraffin-embedded Human endometrium by immunohistochemistry.

Western blot - Anti-GABPB2/Nuclear respiratory factor 2 antibody (AB88746)
  • WB

Unknown

Western blot - Anti-GABPB2/Nuclear respiratory factor 2 antibody (AB88746)

All lanes:

Western blot - Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/mL

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 42 kDa

false

Western blot - Anti-GABPB2/Nuclear respiratory factor 2 antibody (AB88746)
  • WB

Unknown

Western blot - Anti-GABPB2/Nuclear respiratory factor 2 antibody (AB88746)

All lanes:

Western blot - Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/mL

All lanes:

HeLa nuclear extracts at 50 µg

Predicted band size: 42 kDa

false

Western blot - Anti-GABPB2/Nuclear respiratory factor 2 antibody (AB88746)
  • WB

Unknown

Western blot - Anti-GABPB2/Nuclear respiratory factor 2 antibody (AB88746)

All lanes:

Western blot - Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/mL

All lanes:

PC-12 cell lysate at 50 µg

Predicted band size: 42 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GABPB1.

Q06547

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nuclear respiratory factor 2 (NRF2) also known as GABP (GA-binding protein) is a transcription factor that regulates the expression of genes involved in cellular energy metabolism and mitochondrial biogenesis. NRF2 is composed of several subunits with GABPA as its DNA-binding subunit having a molecular mass approximately of 50 kDa. This protein is expressed in various tissues with higher concentrations found in metabolic active tissues such as the brain and muscle. It plays a significant role in controlling the transcription of genes tied to mitochondrial function and overall cellular energy homeostasis.
Biological function summary

NRF2 interacts with other protein factors to initiate and regulate transcription. It functions as part of the GA-binding protein complex interacting with other transcription factors to control gene expression. This regulatory mechanism is vital for cellular adaptation to changing energy demands and influences oxidative stress responses. NRF2 is actively involved in the induction of antioxidant enzymes and proteins that protect the cell from oxidative damage contributing to cellular longevity and function.

Pathways

NRF2 plays an important role in the oxidative stress response pathway and mitochondrial biogenesis. It interacts with other proteins like KEAP1 which regulates NRF2 stability and activity. In the oxidative stress response NRF2 translocates to the nucleus to activate genes associated with antioxidant responses whereas in mitochondrial biogenesis it influences the expression of genes involved in mitochondrial replication and energy production. These pathways are critical for maintaining cellular health and energy supply in response to metabolic demands.

NRF2 has links to neurodegenerative conditions and certain cancer types. In neurodegenerative diseases such as Alzheimer's impaired NRF2 function can lead to increased oxidative stress and neuronal damage. Altered NRF2 signaling is also implicated in some cancers where its role in regulating cell growth and survival becomes dysregulated. NRF2's interaction with KEAP1 is significant in both areas; mutations or changes in KEAP1 can affect NRF2 levels and activity contributing to disease progression and resistance to therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor capable of interacting with purine rich repeats (GA repeats) (PubMed : 10675337, PubMed : 8441384, PubMed : 8816484). Acts as a master regulator of nuclear-encoded mitochondrial genes (By similarity).. (Microbial infection) Necessary for the expression of the Adenovirus E4 gene.
See full target information GABPB1

Alternative Names

E4TF1B, GABPB, GABPB2, GABPB1, GA-binding protein subunit beta-1, GABP subunit beta-1, GABPB-1, GABP subunit beta-2, Nuclear respiratory factor 2, Transcription factor E4TF1-47, Transcription factor E4TF1-53, GABPB-2
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Publications (3)

Recent publications for all applications. Explore the full listand refine your search

Molecular medicine (Cambridge, Mass.) 30:246 PubMed39701936

2024

Depressed TFAM promotes acetaminophen-induced hepatotoxicity regulated by DDX3X-PGC1α-NRF2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Chen,Yaling Cao,Zihao Fan,Ling Xu,Zhenzhen Pan,Yao Gao,Linlin Wei,Qiaoxin Wei,Yuan Tian,Xiangying Zhang,Mei Liu,Feng Ren

Nature communications 13:653 PubMed35115527

2022

TAZ links exercise to mitochondrial biogenesis via mitochondrial transcription factor A.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ha Hwang,Kyung Min Kim,Ho Taek Oh,Gi Don Yoo,Mi Gyeong Jeong,Hyun Lee,Joori Park,Kwon Jeong,Yoon Ki Kim,Young-Gyu Ko,Eun Sook Hwang,Jeong-Ho Hong

Scientific reports 5:14664 PubMed26419333

2015

Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR).

Applications

Unspecified application

Species

Unspecified reactive species

Xavier Gallart-Palau,Aida Serra,Andrew See Weng Wong,Sara Sandin,Mitchell K P Lai,Christopher P Chen,Oi Lian Kon,Siu Kwan Sze
View all publications
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Product promise

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