Goat Polyclonal GABRA4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GABRA4 aa 300-400.
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Publications
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- Alcoholism, clinical and experimental research 43:822-8322019Chronic Ethanol Exposure and Withdrawal Impair Synaptic GABA Receptor-Mediated Neurotransmission in Deep-Layer Prefrontal Cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin A Hughes et. al.PubMed 30860602
- Neuropharmacology 105:124-1322016Differential regulation of synaptic and extrasynaptic α4 GABA(A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn Peyton Bohnsack et. al.PubMed 26767953
- Alcoholism, clinical and experimental research 39:262-712015Chronic intermittent ethanol exposure produces persistent anxiety in adolescent and adult rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCandice E Van Skike,Jaime L Diaz-Granados,Douglas B MatthewsPubMed 25684048
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GABRA4 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link P48169
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-0.30000 µg/mL | Notes - |
Associated Products
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Target data
Function
Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:35355020). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed:35355020). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed:35355020). GABAARs containing alpha-4 are predominantly extrasynaptic, contributing to tonic inhibition in dentate granule cells and thalamic relay neurons (By similarity). Extrasynaptic alpha-4-containing GABAARs control levels of excitability and network activity (By similarity). GABAAR containing alpha-4-beta-3-delta subunits can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness (PubMed:35355020).
Alternative names
Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4, GABAAR subunit alpha-4, GABRA4
Recommended products
Goat Polyclonal GABRA4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GABRA4 aa 300-400.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GABRA4 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link P48169
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-GABRA4 antibody (ab117080)
All lanes: Western blot - Anti-GABRA4 antibody (ab117080) at 0.1 µg/mL
All lanes: Human Frontal Cortex lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRA4 antibody (ab117080)
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab117080 followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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