Anti-GABRD antibody [EPR25324-253] (BSA and Azide free)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control : no staining in mouse kidney. The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse thalamus tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in mouse thalamus (PMID : 23337532). The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum (fresh) tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 (0.956 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml) (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : PBS was used instead of primary antibody, followed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2 μg/ml).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum (fresh) tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 (0.956 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml) (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : PBS was used instead of primary antibody, followed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2 μg/ml).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in rat brain. The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat thalamus tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in rat thalamus. The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in rat cerebellum. The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Gamma-aminobutyric acid receptor subunit delta with ab300348 at 1/500 dilution (0.956 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining in rat cerebellum. The section was incubated with ab300348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

• IP

Supplier Data

Immunoprecipitation - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation.

Gamma-aminobutyric acid receptor subunit delta was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate 10 ug with ab300348 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300348 at 1/1000 dilution.

VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-GABRD antibody [EPR25324-253] (<a href='/en-us/products/primary-antibodies/gabrd-antibody-epr25324-253-ab300348'>ab300348</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate

Lane 2:

<a href='/en-us/products/primary-antibodies/gabrd-antibody-epr25324-253-ab300348'>ab300348</a> at 1/30 IP in mouse cerebellum tissue lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/gabrd-antibody-epr25324-253-ab300348'>ab300348</a> in mouse cerebellum tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 62 kDa

false

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation.

Gamma-aminobutyric acid receptor subunit delta was immunoprecipitated from 0.35 mg Rat cerebellum tissue lysate 10 ug with ab300348 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300348 at 1/1000 dilution.

VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-GABRD antibody [EPR25324-253] (<a href='/en-us/products/primary-antibodies/gabrd-antibody-epr25324-253-ab300348'>ab300348</a>) at 1/1000 dilution

Lane 1:

Rat cerebellum tissue lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/gabrd-antibody-epr25324-253-ab300348'>ab300348</a> at 1/30 IP in rat cerebellum tissue lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/gabrd-antibody-epr25324-253-ab300348'>ab300348</a> in rat cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 62 Da

false

Exposure time: 41s

• WB

Supplier Data

Western blot - Anti-GABRD antibody [EPR25324-253] (BSA and Azide free) (AB300351)

This data was developed using ab300348, the same antibody clone in a different buffer formulation. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 18408071). Negative control : kidney (PMID : 12119096) Samples are non-boiled as boiling may cause protein aggregation. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-GABRD antibody [EPR25324-253] (<a href='/en-us/products/primary-antibodies/gabrd-antibody-epr25324-253-ab300348'>ab300348</a>) at 1/1000 dilution

Lane 1:

Mouse cerebral cortex tissue lysate

Lane 2:

Mouse cerebellum tissue lysate

Lane 3:

Mouse kidney tissue lysate

Lane 4:

Rat cerebellum tissue lysate

Lane 5:

Rat kidney tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 62 kDa

false

Exposure time: 5.5s