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AB93619

Anti-GABRD antibody [N151/3]

3

(2 Reviews)

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(3 Publications)

Mouse Monoclonal GABRD antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Rat Gabrd aa 1-50.

View Alternative Names

Gamma-aminobutyric acid receptor subunit delta, GABA(A) receptor subunit delta, GABAAR subunit delta, Gabrd

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GABRD antibody [N151/3] (AB93619)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABRD antibody [N151/3] (AB93619)

Cold 100% methanol for 10 minutes at -20°C fixed human HaCaT cells labeling GABRD (green) using ab93619 at 1/10 dilution in ICC/IF.

FITC Goat Anti-Mouse was used as the secondary antibody at 1/50 dilution for 1 hour at room temperature. STaining in the diffuse cytoplasm and dull nuclei is observed.

Immunocytochemistry/ Immunofluorescence - Anti-GABRD antibody [N151/3] (AB93619)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABRD antibody [N151/3] (AB93619)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling GABRD with ab93619 at 1/100 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Delta Antibody (D) Composite.

Western blot - Anti-GABRD antibody [N151/3] (AB93619)
  • WB

Supplier Data

Western blot - Anti-GABRD antibody [N151/3] (AB93619)

Block : 15% BSA for 30 minutes at RT

All lanes:

Western blot - Anti-GABRD antibody [N151/3] (ab93619) at 1/1000 dilution

All lanes:

Rat cell line lysates at 15 µg

Secondary

All lanes:

Sheep Anti-Mouse IgG HRP

Predicted band size: 51 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N151/3

Isotype

IgG2a

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Rat Gabrd aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P18506

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

The clone number has been updated from S151-3 to N151/3, both clone numbers name the same antibody clone.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Delta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (By similarity). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (By similarity). GABAARs containing delta/GABRD subunits are predominantly expressed and located in extrasynaptic or perisynaptic positions on hippocampus and cerebellar granule cells, and contribute to the tonic GABAergic inhibition (PubMed : 9464994). GABAAR containing alpha-4-beta-3-delta subunits can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness (By similarity).
See full target information Gabrd
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1202725 PubMed37465666

2023

Prognostic and immunological significance of an M1 macrophage-related gene signature in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Mao,Fanglong Song,Ju Jin,Bin Zou,Peijun Dai,Mingjuan Sun,Weicheng Xu,Lianghua Wang,Yifan Kang

Journal of neurophysiology 126:637-652 PubMed34259044

2021

Diurnal properties of tonic and synaptic GABA receptor-mediated currents in suprachiasmatic nucleus neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Moldavan,Olga Cravetchi,Charles N Allen

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences 49:439-446 PubMed32985156

2020

[Correlation between transient receptor potential canonical channel with heart and kidney injure of rat model of obstructive sleep apnea hypopnea syndrome].

Applications

Unspecified application

Species

Unspecified reactive species

Wen Wen,Qiaoling Yao,Yulan Chen,Zhiqiang Li,Xiaojing Sun,Yu Li,Junshi Zhang,Zhulipiya Simayi,Xinjuan Xu
View all publications
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