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AB325669

Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric)

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Chicken Monoclonal antibody. Suitable for IHC-P, WB and reacts with Rat samples.

View Alternative Names

Gad67, Gad1, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD-67

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum stained with ab325669 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield mounting medium. DAPI (blue) shows cell nuclei. The antibody stains neuropil granules identifying GABAergic synapses, Purkinje cell bodies, and the synaptic terminal of GABAergic interneurons in the molecular layer of the cerebellum. Magnification 400X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum stained with ab325669 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield mounting medium. DAPI (blue) shows cell nuclei. The antibody stains neuropil granules identifying GABAergic synapses, Purkinje cell bodies, and the synaptic terminal of GABAergic interneurons in the molecular layer of the cerebellum. Magnification 100X.

Western blot - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)
  • WB

Supplier Data

Western blot - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)

Western blotting of rat brain homogenate with an anti-GAD65/67 mouse mAb and ab325669. The mouse mAb and ab325669 were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. The antibody recognizes endogenous GAD1 with similar sensitivity as the anti-GAD65/67 mouse Mab in the rat brain homogenate at 65-67 kDa. GAD1 is an alternative name for GAD65/67.

All lanes:

Western blot - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (ab325669) at 10 µg/mL

All lanes:

rat brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 65-67 kDa

false

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

L127/12

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Cross-reacts with GAD25, GAD44 and GAD65/GAD2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L127/12 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L127/12 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor.
See full target information Gad1
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Product promise

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For full details, please see our Terms & Conditions

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