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Mouse Monoclonal GAD65 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 76 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Gad2.


Images

Western blot - Anti-GAD65 antibody [GAD-6] (AB26113), expandable thumbnail
  • Western blot - Anti-GAD65 antibody [GAD-6] (AB26113), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Full Length Protein corresponding to Rat Gad2. The exact immunogen used to generate this antibody is proprietary information. Database link Q05683

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Mammals
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/20000
Notes

-

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mammals
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Catalyzes the production of GABA.

Alternative names

Gad65, Gad2, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform, GAD-65

Recommended products

Mouse Monoclonal GAD65 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 76 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Gad2.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Full Length Protein corresponding to Rat Gad2. The exact immunogen used to generate this antibody is proprietary information. Database link Q05683
Clone number
GAD-6
Purification technique
Affinity purification Protein A/G
Specificity

The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991).

Epitope
ab26113 recognizes an epitope near the C-terminus of GAD65
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-GAD65 antibody [GAD-6] (ab26113), expandable thumbnail

    Western blot - Anti-GAD65 antibody [GAD-6] (ab26113)

    All lanes: Western blot - Anti-GAD65 antibody [GAD-6] (ab26113) at 1/1000 dilution

    All lanes: mouse cerebellum at 0.75 µg

    Secondary

    All lanes: HRP horse anti-mouse IgG at 1/5000 dilution

    Predicted band size: 65 kDa

  • Western blot - Anti-GAD65 antibody [GAD-6] (ab26113), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-GAD65 antibody [GAD-6] (ab26113)

    GAD65 western blot using anti-GAD65 antibody [GAD-6] ab26113. Publication image and figure legend from Woodhouse, A., Fernández-Martos, C. M., et al., 2018, BMC Anesthesiol, PubMed 29699479.


    ab26113 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab26113 please see the product overview.

    No change in synaptic proteins between propofol and vehicle treated APP/PS1 (n = 3 and 2) and control wild-type (n = 3 for both) mice. Western blot analysis (a) showed no robust change in the expression levels of PSD-95, GAD65/67 and synaptophysin (SYN) in wild-type or APP/PS1 mice treated multiple times with propofol (Prop) or vehicle (VH). Bar graphs show the mean intensity ± SEM of PSD-95 (b), GAD65/67 (c) and synaptophysin (d) normalized to GAPDH

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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