AB26113

Anti-GAD65 antibody [GAD-6]

Name: Anti-GAD65 antibody [GAD-6] (ab26113) | Abcam
Brand: Abcam Limited
SKU: AB26113
Price: 500 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(89 Publications)

Anti-GAD65 antibody [GAD-6] (ab26113) is a mouse monoclonal antibody detecting GAD65 in Western Blot. Suitable for Human, Mouse.

- Over 70 publications
- Trusted since 2005

View Alternative Names

Gad65, Gad2, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform, GAD-65

2 Images

Supplier Data

Western blot - Anti-GAD65 antibody [GAD-6] (AB26113)

All lanes:

Western blot - Anti-GAD65 antibody [GAD-6] (ab26113) at 1/1000 dilution

All lanes:

mouse cerebellum at 0.75 µg

Secondary

All lanes:

HRP horse anti-mouse IgG at 1/5000 dilution

Predicted band size: 65 kDa

false

CiteAb

Western blot - Anti-GAD65 antibody [GAD-6] (AB26113)

GAD65 western blot using anti-GAD65 antibody [GAD-6] ab26113. Publication image and figure legend from Woodhouse, A., Fernández-Martos, C. M., et al., 2018, BMC Anesthesiol, PubMed 29699479.

ab26113 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab26113 please see the product overview.

No change in synaptic proteins between propofol and vehicle treated APP/PS1 (n = 3 and 2) and control wild-type (n = 3 for both) mice. Western blot analysis (a) showed no robust change in the expression levels of PSD-95, GAD65/67 and synaptophysin (SYN) in wild-type or APP/PS1 mice treated multiple times with propofol (Prop) or vehicle (VH). Bar graphs show the mean intensity ± SEM of PSD-95 (b), GAD65/67 (c) and synaptophysin (d) normalized to GAPDH

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GAD-6

Isotype

IgG2a

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Full Length Protein corresponding to Rat Gad2. The exact immunogen used to generate this antibody is proprietary information.

Q05683

Epitope

ab26113 recognizes an epitope near the C-terminus of GAD65

Specificity

The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991).

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-GAD65 antibody [GAD-6] (ab26113) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.

What is the molecular weight of GAD65?
Anti-GAD65 [GAD-6] (ab26113) specifically detects a band for GAD65 (UniProt: Q05329) at a molecular weight of 65kDa.

Trusted by the scientific community
Anti-GAD65 [GAD-6] (ab26113) was first used in a scientific publication in 2005 and has been cited over 70 times in peer-reviewed journals.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the production of GABA.

See full target information Gad2

style

left-column

Publications (89)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:194 PubMed40713786

2025

Coexistence of CN1A autoantibodies in GAD65 encephalitis exacerbates neurodegeneration : Novel autoantibodies in GAD65 encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Breuer,Delara Kamalizade,Tobias Baumgartner,Juliane L Berns,Thoralf Opitz,Franziska S Thaler,Susanne Schoch,Lars Komorowski,Christoph Helmstaedter,Rainer Surges,Albert J Becker,Julika Pitsch

Pain reports 9:e1209 PubMed39512583

2024

Upregulated spinal histone deacetylases induce nociceptive sensitization by inhibiting the GABA system in chronic constriction injury-induced neuropathy in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Hong Wen,Nan-Fu Chen,Hao-Jung Cheng,Hsiao-Mei Kuo,Pei-Yu Chen,Chien-Wei Feng,Zhi-Kang Yao,Wu-Fu Chen,Chun-Sung Sung

The Journal of biological chemistry 300:107779 PubMed39276933

2024

Downregulation of chloride voltage-gated channel 7 contributes to hyperalgesia following spared nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yunyun Cai,Jiajie Li,Kewei Fan,Dongmei Zhang,Hongjian Lu,Gang Chen

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 21:e00445 PubMed39242290

2024

Extracellular vesicles from mesenchymal stem cells alter gut microbiota and improve neuroinflammation and motor impairment in rats with mild liver damage.

Applications

Unspecified application

Species

Unspecified reactive species

Gergana Mincheva,Vicente Felipo,Victoria Moreno-Manzano,Alfonso Benítez-Páez,Marta Llansola

The journal of venomous animals and toxins including tropical diseases 30:e20230065 PubMed38770186

2024

Effects of local and systemic treatment with human natural killer-1 mimetic peptide (HNK-1) after ventral root avulsion and reimplantation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Scanavachia da Silva,Julia Lombardi,Frank Kirchhoff,Rui Seabra Ferreira,Benedito Barraviera,Alexandre Leite Rodrigues de Oliveira,Luciana Politti Cartarozzi

Frontiers in immunology 14:1213710 PubMed37954604

2023

Electroacupuncture exerts prolonged analgesic and neuroprotective effects in a persistent dental pain model induced by multiple dental pulp injuries: GABAergic interneurons-astrocytes interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Sharmely Sharon Ballon Romero,Lih-Jyh Fuh,Shih-Ya Hung,Yu-Chen Lee,Yu-Chuen Huang,Szu-Yu Chien,Yi-Hung Chen

Frontiers in cellular neuroscience 17:1211486 PubMed37711512

2023

Neuroprotection by upregulation of the major histocompatibility complex class I (MHC I) in SOD1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Laura M R Tomiyama,Luciana Politti Cartarozzi,Lilian de Oliveira Coser,Gabriela Bortolança Chiarotto,Alexandre L R Oliveira

Behavioural brain research 448:114442 PubMed37085118

2023

Evaluations of memory, anxiety, and the growth factor IGF-1R after post-surgical menopause treatment with a highly selective progestin.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria E Bernaud,Stephanie V Koebele,Steven N Northup-Smith,Mari N Willeman,Charlotte Barker,Alex Schatzki-Lumpkin,Maria Valenzuela Sanchez,Heather A Bimonte-Nelson

Biomolecules 13: PubMed36671571

2023

Developmental Inhibitory Changes in the Primary Somatosensory Cortex of the Stargazer Mouse Model of Absence Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Hassan,David R Grattan,Beulah Leitch

Nature communications 13:7805 PubMed36528681

2022

Closed-loop direct control of seizure focus in a rodent model of temporal lobe epilepsy via localized electric fields applied sequentially.

Applications

Unspecified application

Species

Unspecified reactive species

Wonok Kang,Chanyang Ju,Jaesoon Joo,Jiho Lee,Young-Min Shon,Sung-Min Park

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAD65 antibody [GAD-6]

Western blot - Anti-GAD65 antibody [GAD-6] (AB26113)

Western blot - Anti-GAD65 antibody [GAD-6] (AB26113)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (89)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise