Rabbit Polyclonal GAD65 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human GAD1 aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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- International journal of molecular sciences 23:2022Axonal Tract Reconstruction Using a Tissue-Engineered Nigrostriatal Pathway in a Rat Model of Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura A Struzyna,Kevin D Browne,Justin C Burrell,Wisberty J Gordián Vélez,Kathryn L Wofford,Hilton M Kaplan,N Sanjeeva Murthy,H Isaac Chen,John E Duda,Rodrigo A España,D Kacy CullenPubMed 36430464
- EMBO molecular medicine 14:e147972022Parkinson's disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica Giehrl-Schwab,Florian Giesert,Benedict Rauser,Chu Lan Lao,Sina Hembach,Sandrine Lefort,Ignacio L Ibarra,Christina Koupourtidou,Malte Daniel Luecken,Dong-Jiunn Jeffery Truong,Judith Fischer-Sternjak,Giacomo Masserdotti,Nilima Prakash,Jovica Ninkovic,Sabine M Hölter,Daniela M Vogt Weisenhorn,Fabian J Theis,Magdalena Götz,Wolfgang WurstPubMed 35373464
- Scientific reports 11:221672021Partial protective effects of melatonin on developing brain in a rat model of chorioamnionitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeraldine Favrais,Elie Saliba,Léa Savary,Sylvie Bodard,Zuhal Gulhan,Pierre Gressens,Sylvie ChalonPubMed 34773065
- Nature metabolism 2:243-2552020PTH hypersecretion triggered by a GABA and Ca-sensing receptor heterocomplex in hyperparathyroidism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenhan Chang et. al.PubMed 32694772
- Current biology : CB 30:196-208.e82020Dopamine Signaling in the Suprachiasmatic Nucleus Enables Weight Gain Associated with Hedonic Feeding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan M Grippo,Qijun Tang,Qi Zhang,Sean R Chadwick,Yingnan Gao,Everett B Altherr,Laura Sipe,Aarti M Purohit,Nidhi M Purohit,Meghana D Sunkara,Krystyna J Cios,Michael Sidikpramana,Anthony J Spano,John N Campbell,Andrew D Steele,Jay Hirsh,Christopher D Deppmann,Martin Wu,Michael M Scott,Ali D GülerPubMed 31902720
- Scientific reports 9:174022019Sonic Hedgehog is expressed by hilar mossy cells and regulates cellular survival and neurogenesis in the adult hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuis E Gonzalez-Reyes et. al.PubMed 31758070
- Translational psychiatry 9:2642019Region specific knockdown of Parvalbumin or Somatostatin produces neuronal and behavioral deficits consistent with those observed in schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephanie M Perez et. al.PubMed 31636253
- Journal of anatomy 235:569-5892019Cajal-Retzius neurons are required for the development of the human hippocampal fissure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGundela Meyer,Emilio González-Arnay,Ute Moll,Alice Nemajerova,Fadel Tissir,Miriam González-GómezPubMed 30861578
- Nature communications 10:9732019Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson's disease pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIria Carballo-Carbajal et. al.PubMed 30846695
- Scientific reports 8:176862018Loss of interneurons and disruption of perineuronal nets in the cerebral cortex following hypoxia-ischaemia in near-term fetal sheep.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTania M Fowke et. al.PubMed 30523273
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GAD1 aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q99259
Consider this alternative
Reactivity data
Select an application| IHC-P | |
|---|---|
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/2000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Additional Targets
Gad1
Alternative names
Gad65, Gad2, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform, GAD-65
Recommended products
Rabbit Polyclonal GAD65 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human GAD1 aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GAD1 aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q99259
- Purification technique
- Ion exchange chromatography
- Concentration
- Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other Rabbit serum proteins.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (ab49832)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat pancreatics sections labelling GAD65 & GAD67 with ab49832 at a concentration of 1/1000. The secondary used was a biotin peroxidase.
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (ab49832)
ab49832 staining human pancreas tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in 10mM citrate buffer prior to incubation with ab49832, diluted 1/2000, for 1 hour at 25°C. An Alexa Fluor® 546 conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
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