AB49832

Anti-GAD65 + GAD67 antibody

Name: Anti-GAD65 + GAD67 antibody (ab49832) | Abcam
Brand: Abcam Limited
SKU: AB49832
Price: 595 USD
Availability: InStock
Rating: 3 (6 reviews)

(6 Reviews)

(34 Publications)

Rabbit Polyclonal GAD65 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 34 publications. Immunogen corresponding to Synthetic Peptide within Human GAD1 aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Gad65, Gad2, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform, GAD-65

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (AB49832)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (AB49832)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat pancreatics sections labelling GAD65 & GAD67 with ab49832 at a concentration of 1/1000. The secondary used was a biotin peroxidase.

IHC-P

AbReview10864****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (AB49832)

ab49832 staining human pancreas tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in 10mM citrate buffer prior to incubation with ab49832, diluted 1/2000, for 1 hour at 25°C. An Alexa Fluor® 546 conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human GAD1 aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q99259

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other Rabbit serum proteins.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the production of GABA.

See full target information Gad2

Additional targets

Gad1

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Brain structure & function 230:96 PubMed40498232

2025

Developmental changes of Reelin-expressing cell populations in the marginal zone of the neocortex of the European wild boar, Sus scrofa.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Sobierajski,Miriam González-Gómez,Emilio González-Arnay,Petra Wahle,Gundela Meyer

Nature communications 15:7922 PubMed39256428

2024

A distinct neuronal ensemble of prelimbic cortex mediates spontaneous pain in rats with peripheral inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Longyu Ma,Lupeng Yue,Shuting Liu,Shi Xu,Jifu Tong,Xiaoyan Sun,Li Su,Shuang Cui,Feng-Yu Liu,You Wan,Ming Yi

Cells 13: PubMed39056776

2024

Human-Induced Pluripotent Stem Cell (iPSC)-Derived GABAergic Neuron Differentiation in Bipolar Disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J Schill,Durga Attili,Cynthia J DeLong,Melvin G McInnis,Craig N Johnson,Geoffrey G Murphy,K Sue O'Shea

Advanced healthcare materials 12:e2201720 PubMed36447307

2022

Delivery of Induced Neural Stem Cells Through Mechano-Tuned Silk-Collagen Hydrogels for the Recovery of Contused Spinal Cord in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ganchimeg Davaa,Jin Young Hong,Jung-Hwan Lee,Min Soo Kim,Jennifer O Buitrago,Yu-Meng Li,Hae-Hyoung Lee,Dong Wook Han,Kam W Leong,Jung Keun Hyun,Hae-Won Kim

International journal of molecular sciences 23: PubMed36430464

2022

Axonal Tract Reconstruction Using a Tissue-Engineered Nigrostriatal Pathway in a Rat Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Laura A Struzyna,Kevin D Browne,Justin C Burrell,Wisberty J Gordián Vélez,Kathryn L Wofford,Hilton M Kaplan,N Sanjeeva Murthy,H Isaac Chen,John E Duda,Rodrigo A España,D Kacy Cullen

EMBO molecular medicine 14:e14797 PubMed35373464

2022

Parkinson's disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Giehrl-Schwab,Florian Giesert,Benedict Rauser,Chu Lan Lao,Sina Hembach,Sandrine Lefort,Ignacio L Ibarra,Christina Koupourtidou,Malte Daniel Luecken,Dong-Jiunn Jeffery Truong,Judith Fischer-Sternjak,Giacomo Masserdotti,Nilima Prakash,Jovica Ninkovic,Sabine M Hölter,Daniela M Vogt Weisenhorn,Fabian J Theis,Magdalena Götz,Wolfgang Wurst

Scientific reports 11:22167 PubMed34773065

2021

Partial protective effects of melatonin on developing brain in a rat model of chorioamnionitis.

Applications

Unspecified application

Species

Unspecified reactive species

Geraldine Favrais,Elie Saliba,Léa Savary,Sylvie Bodard,Zuhal Gulhan,Pierre Gressens,Sylvie Chalon

Nature metabolism 2:243-255 PubMed32694772

2020

PTH hypersecretion triggered by a GABA and Ca-sensing receptor heterocomplex in hyperparathyroidism.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhan Chang,Chia-Ling Tu,Frederic G Jean-Alphonse,Amanda Herberger,Zhiqiang Cheng,Jenna Hwong,Hanson Ho,Alfred Li,Dawei Wang,Hongda Liu,Alex D White,Insoo Suh,Wen Shen,Quan-Yang Duh,Elham Khanafshar,Dolores M Shoback,Kunhong Xiao,Jean-Pierre Vilardaga

Current biology : CB 30:196-208.e8 PubMed31902720

2020

Dopamine Signaling in the Suprachiasmatic Nucleus Enables Weight Gain Associated with Hedonic Feeding.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan M Grippo,Qijun Tang,Qi Zhang,Sean R Chadwick,Yingnan Gao,Everett B Altherr,Laura Sipe,Aarti M Purohit,Nidhi M Purohit,Meghana D Sunkara,Krystyna J Cios,Michael Sidikpramana,Anthony J Spano,John N Campbell,Andrew D Steele,Jay Hirsh,Christopher D Deppmann,Martin Wu,Michael M Scott,Ali D Güler

Scientific reports 9:17402 PubMed31758070

2019

Sonic Hedgehog is expressed by hilar mossy cells and regulates cellular survival and neurogenesis in the adult hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Luis E Gonzalez-Reyes,Chia-Chu Chiang,Mingming Zhang,Joshua Johnson,Manuel Arrillaga-Tamez,Nicholas H Couturier,Neha Reddy,Lev Starikov,Jeffrey R Capadona,Andreas H Kottmann,Dominique M Durand

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAD65 + GAD67 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (AB49832)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody (AB49832)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Additional targets

Publications (34)

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