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Anti-GAD65 + GAD67 antibody [EPR19366] is a rabbit monoclonal antibody that is used in GAD65/67 western blot (WB), immunoprecipitation (IP), immunocytochemistry/immunofluorescence (ICC/IF), immunohistochemistry on frozen sections (IHC-Fr), and immunohistochemistry on paraffin-embedded sections (IHC-P). Suitable for human, mouse and rat samples.

- Cited in over 15 publications
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Affinity purified using Protein A


Images

Immunocytochemistry/ Immunofluorescence - Anti-GAD65 + GAD67 antibody [EPR19366] (AB183999), expandable thumbnail
  • Immunoprecipitation - Anti-GAD65 + GAD67 antibody [EPR19366] (AB183999), expandable thumbnail
  • Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (AB183999), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (AB183999), expandable thumbnail
  • Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (AB183999), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-FrIHC-P
Human
Tested
Tested
Expected
Expected
Expected
Mouse
Expected
Tested
Tested
Tested
Tested
Rat
Expected
Tested
Expected
Expected
Tested
Common marmoset
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/40

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Predicted
Predicted

Species

Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2500

Notes

-

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Common marmoset

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the production of GABA.

Targets

GAD1

Alternative names

Recommended products

Anti-GAD65 + GAD67 antibody [EPR19366] is a rabbit monoclonal antibody that is used in GAD65/67 western blot (WB), immunoprecipitation (IP), immunocytochemistry/immunofluorescence (ICC/IF), immunohistochemistry on frozen sections (IHC-Fr), and immunohistochemistry on paraffin-embedded sections (IHC-P). Suitable for human, mouse and rat samples.

- Cited in over 15 publications
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Affinity purified using Protein A

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19366

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling GAD65 + GAD67 with purified ab183999 at 1/100 (10 μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 antibody [HM-2]; Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

  • Immunoprecipitation - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Immunoprecipitation - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    ab183999 Immunoprecipitating GAD65 + GAD67 in Human cerebellum lysate. 10μg of cell lysate was incubated with primary antibody 1/40. For western blotting ab183999 (1/1000) was used to confirm successful immunoprecipation. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000.

    Lane 1 (Input): Human cerebellum lysate 10μg
    Lane 2 (+): Human cerebellum lysate with ab183999, 1/40
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab183999 in Human cerebellum lysate

    All lanes: Immunoprecipitation - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Predicted band size: 65 kDa

    Exposure time: 2min

  • Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2: 15 seconds.

    Mouse GAD67 fragment recombinant protein contain aa468-592 with a His-Tag®. Mouse GAD65 fragment recombinant protein contain aa460-584 with a His-Tag®. These two fragment recombinant proteins were made in-house. The ~30 kDa band represents doublets of the recombinant fragments.

    All lanes: Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999) at 1/1000 dilution

    Lane 1: Mouse GAD67 fragment recombinant protein at 0.01 µg

    Lane 2: Mouse GAD65 fragment recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling GAD65 + GAD67 with ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on mouse cerebellum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-5: 3 minutes; Lane 6 and 7: 2 seconds.

    All lanes: Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999) at 1/1000 dilution

    Lane 1: Human cerebellum lysate at 20 µg

    Lane 2: Mouse skin lysate at 20 µg

    Lane 3: Mouse lung lysate at 20 µg

    Lane 4: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

    Lane 5: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

    Lane 6: Mouse cerebellum lysate at 20 µg

    Lane 7: Rat cerebellum lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa

    Observed band size: 65 kDa, 67 kDa

  • Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lane 1: Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999) at 1/5000 dilution

    Lane 2: Western blot - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Rat brain lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa

    Observed band size: 65 kDa, 67 kDa

    Exposure time: 2s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling GAD65 + GAD67 with ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on mouse pancreas islets is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling GAD65 + GAD67 with ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative on mouse lung. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permealized frozen section of Mouse hippocampus tissue labeling GAD65 + GAD67 with ab183999 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). The result showed mainly cytoplasmic staining on mouse hippocampus. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling GAD65 + GAD67 with ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on rat cerebellum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling GAD65 + GAD67 with ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on rat pancreas islet is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] (ab183999)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling GAD65 + GAD67 with ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative on rat kidney. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

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