Rabbit Recombinant Monoclonal GAD65 antibody. Carrier free. Suitable for IP, ICC/IF, IHC-Fr, IHC-P, WB and reacts with Human, Mouse, Rat, Recombinant fragment - Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (AB240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunocytochemistry-immunofluorescence-img49122.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (AB240280)")
![Immunoprecipitation - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (AB240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunoprecipitation-img3301.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (AB240280)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (AB240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84798.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (AB240280)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (AB240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132766.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (AB240280)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (AB240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132833.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (AB240280)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Tested |
| Mouse | Expected | Tested | Tested | Tested | Tested |
| Rat | Expected | Expected | Expected | Tested | Tested |
| Recombinant fragment - Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
2 products for Alternative Version
Additional Targets
GAD1
Alternative names
GAD65, GAD2, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform, GAD-65
Recommended products
Rabbit Recombinant Monoclonal GAD65 antibody. Carrier free. Suitable for IP, ICC/IF, IHC-Fr, IHC-P, WB and reacts with Human, Mouse, Rat, Recombinant fragment - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19366
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240280 is the carrier-free version of Anti-GAD65 + GAD67 antibody [EPR19366] ab183999.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunocytochemistry/ Immunofluorescence - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunocytochemistry-immunofluorescence-img49122.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
Immunocytochemistry/ Immunofluorescence - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)
Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling GAD65 + GAD67 with purified Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 at 1/100 (10 μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 antibody [HM-2]; Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GAD65 + GAD67 antibody [EPR19366] ab183999).![Immunoprecipitation - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunoprecipitation-img3301.jpg "Immunoprecipitation - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
Immunoprecipitation - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)
Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 Immunoprecipitating GAD65 + GAD67 in Human cerebellum lysate. 10μg of cell lysate was incubated with primary antibody 1/40. For western blotting Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 (1/1000) was used to confirm successful immunoprecipation. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/5000 dilution.
Lane 1 (Input): Human cerebellum lysate 10μg
Lane 2 (+): Human cerebellum lysate with Anti-GAD65 + GAD67 antibody [EPR19366] ab183999, 1/40
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 in Human cerebellum lysateThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GAD65 + GAD67 antibody [EPR19366] ab183999).
All lanes: Immunoprecipitation - Anti-GAD65 + GAD67 antibody [EPR19366] (Anti-GAD65 + GAD67 antibody [EPR19366] ab183999)
Predicted band size: 65 kDa
Exposure time: 2min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84798.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling GAD65 + GAD67 with Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on mouse cerebellum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GAD65 + GAD67 antibody [EPR19366] ab183999).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132766.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)
Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling GAD65 + GAD67 with Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on mouse pancreas islets is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GAD65 + GAD67 antibody [EPR19366] ab183999).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132833.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling GAD65 + GAD67 with Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative on mouse lung. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GAD65 + GAD67 antibody [EPR19366] ab183999).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-frozen-sections-img70288.jpg "Immunohistochemistry (Frozen sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
Immunohistochemistry (Frozen sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)
Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permealized frozen section of Mouse hippocampus tissue labeling GAD65 + GAD67 with Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). The result showed mainly cytoplasmic staining on mouse hippocampus. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GAD65 + GAD67 antibody [EPR19366] ab183999).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84453.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling GAD65 + GAD67 with Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on rat cerebellum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GAD65 + GAD67 antibody [EPR19366] ab183999).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10203.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)
Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling GAD65 + GAD67 with Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on rat pancreas islet is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GAD65 + GAD67 antibody [EPR19366] ab183999).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127684.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling GAD65 + GAD67 with Anti-GAD65 + GAD67 antibody [EPR19366] ab183999 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative on rat kidney. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GAD65 + GAD67 antibody [EPR19366] ab183999).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-frozen-sections-img70288.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84453.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10203.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280), expandable thumbnail](https://content.abcam.com/products/images/gad65-gad67-antibody-epr19366-bsa-and-azide-free-ab240280--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127684.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD65 + GAD67 antibody [EPR19366] - BSA and Azide free (ab240280)")
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