Rabbit Polyclonal GAD67 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GAD1.
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Publications
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- Journal of oncology 2023:60278602023The Long Noncoding RNA Cytoskeleton Regulator RNA (CYTOR)/miRNA-24-3p Axis Facilitates Nasopharyngeal Carcinoma Progression by Modulating GAD1 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingwei Du,Wangbo Yu,Lijuan Peng,Tao ZhangPubMed 36814556
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GAD1. The exact immunogen used to generate this antibody is proprietary information. Database link Q99259
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Mouse | Tested | Tested | Expected |
| Rat | Expected | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor. Isoform 3. Enzymatically inactive as glutamate decarboxylase.
Alternative names
GAD, GAD67, GAD1, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD-67
Recommended products
Rabbit Polyclonal GAD67 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GAD1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GAD1. The exact immunogen used to generate this antibody is proprietary information. Database link Q99259
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody (ab228710)
GAD67/GAD1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-GAD67/GAD1 antibody
Paraffin-embedded adult mouse retina stained for GAD67 protein (green) using ab228710 at 1/250 dilution in immunohistochemical analysis.
Beta Tubulin 3/TUJ1 (red) stained by beta Tubulin 3/TUJ1 antibody diluted at 1/250.
Nuclear counterstain: Fluoroshield with DAPI (blue).
Western blot - Anti-GAD67/GAD1 antibody (ab228710)
GAD67/GAD1 Western blot staining using rabbit Anti-GAD67/GAD1 antibody
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-GAD67/GAD1 antibody (ab228710) at 1/500 dilution
Lane 1: Mouse brain whole tissue extract at 50 µg
Lane 2: Rat brain whole tissue extract at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 66 kDa
Western blot - Anti-GAD67/GAD1 antibody (ab228710)
GAD67/GAD1 Western blot staining using rabbit Anti-GAD67/GAD1 antibody
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-GAD67/GAD1 antibody (ab228710) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: GAD67-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody (ab228710)
GAD67/GAD1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-GAD67/GAD1 antibody
DIV9 rat E18 primary cortical neurons stained for GAD67 (green) using ab228710 at 1/500 dilution in ICC/IF. Neurons were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Fluoroshield with DAPI (blue).
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