AB228710

Anti-GAD67/GAD1 antibody

Name: Anti-GAD67/GAD1 antibody (ab228710) | Abcam
Brand: Abcam Limited
SKU: AB228710
Price: 525 USD
Availability: InStock
Rating: 2 (2 reviews)

(2 Reviews)

(3 Publications)

Rabbit Polyclonal GAD67 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant fragment, Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GAD1.

View Alternative Names

GAD, GAD67, GAD1, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD-67

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody (AB228710)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody (AB228710)

Paraffin-embedded adult mouse retina stained for GAD67 protein (green) using ab228710 at 1/250 dilution in immunohistochemical analysis.

Beta Tubulin 3/TUJ1 (red) stained by beta Tubulin 3/TUJ1 antibody diluted at 1/250.

Nuclear counterstain : Fluoroshield with DAPI (blue).

Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody (AB228710)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody (AB228710)

DIV9 rat E18 primary cortical neurons stained for GAD67 (green) using ab228710 at 1/500 dilution in ICC/IF. Neurons were fixed in 4% paraformaldehyde at RT for 15 minutes.

Nuclear counterstain : Fluoroshield with DAPI (blue).

Supplier Data

Western blot - Anti-GAD67/GAD1 antibody (AB228710)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-GAD67/GAD1 antibody (ab228710) at 1/500 dilution

Lane 1:

Mouse brain whole tissue extract at 50 µg

Lane 2:

Rat brain whole tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 66 kDa

true

Supplier Data

Western blot - Anti-GAD67/GAD1 antibody (AB228710)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-GAD67/GAD1 antibody (ab228710) at 1/5000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

GAD67 transfected 293T whole cell lysates at 30 µg

Lane 3:

GAD65 transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GAD1. The exact immunogen used to generate this antibody is proprietary information.

Q99259

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor.. Isoform 3. Enzymatically inactive as glutamate decarboxylase.

See full target information GAD1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39337341

2024

Cell-Type-Specific Expression of Leptin Receptors in the Mouse Forebrain.

Applications

Unspecified application

Species

Unspecified reactive species

Cade R Canepa,John A Kara,Charles C Lee

Journal of oncology 2023:6027860 PubMed36814556

2023

The Long Noncoding RNA Cytoskeleton Regulator RNA (CYTOR)/miRNA-24-3p Axis Facilitates Nasopharyngeal Carcinoma Progression by Modulating GAD1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Du,Wangbo Yu,Lijuan Peng,Tao Zhang

Journal of immunology (Baltimore, Md. : 1950) 208:672-684 PubMed35022275

2022

Hepatitis B and Hepatitis C Virus Infection Promote Liver Fibrogenesis through a TGF-β1-Induced OCT4/Nanog Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Li,Xiaoqiong Duan,Chuanlong Zhu,Xiao Liu,Andre J Jeyarajan,Min Xu,Zeng Tu,Qiuju Sheng,Dong Chen,Chuanwu Zhu,Tuo Shao,Zhimeng Cheng,Shadi Salloum,Esperance A Schaefer,Annie J Kruger,Jacinta A Holmes,Raymond T Chung,Wenyu Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAD67/GAD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody (AB228710)

Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody (AB228710)

Western blot - Anti-GAD67/GAD1 antibody (AB228710)

Western blot - Anti-GAD67/GAD1 antibody (AB228710)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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