Mouse monoclonal [K-87] to GAD67/GAD1 is a mouse monoclonal antibody that is used to detect GAD67/GAD1 in Flow cytometry, ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 100 publications
- Trusted since 2005
Images
![Western blot - Anti-GAD67/GAD1 antibody [K-87] (AB26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--western-blot-img153993.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAD67/GAD1 antibody [K-87] (AB26116)")
![Flow Cytometry - Anti-GAD67/GAD1 antibody [K-87] (AB26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--flow-cytometry-img50857.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-GAD67/GAD1 antibody [K-87] (AB26116)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (AB26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110186.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (AB26116)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (AB26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110010.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (AB26116)")
![Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody [K-87] (AB26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--immunocytochemistry-immunofluorescence-img104499.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody [K-87] (AB26116)")
Publications
Filter by
- Nature communications 14:79712023Enhanced TARP-γ8-PSD-95 coupling in excitatory neurons contributes to the rapid antidepressant-like action of ketamine in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShi-Ge Xue,Jin-Gang He,Ling-Li Lu,Shi-Jie Song,Mei-Mei Chen,Fang Wang,Jian-Guo ChenPubMed 38042894
- Nature communications 14:44562023Effects of oxidative stress on hepatic encephalopathy pathogenesis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunhu Bai,Kenan Li,Xiaodong Li,Xiyu Chen,Jie Zheng,Feifei Wu,Jinghao Chen,Ze Li,Shuai Zhang,Kun Wu,Yong Chen,Yayun Wang,Yanling YangPubMed 37488119
- NPJ Regenerative medicine 8:362023MAP4Ks inhibition promotes retinal neuron regeneration from Müller glia in adult mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHoujian Zhang,Yuli Guo,Yaqiong Yang,Yuqian Wang,Youwen Zhang,Jingbin Zhuang,Yuting Zhang,Mei Shen,Jiankai Zhao,Rongrong Zhang,Yan Qiu,Shiying Li,Jiaoyue Hu,Wei Li,Jianfeng Wu,Haiwei Xu,Steven J Fliesler,Yi Liao,Zuguo LiuPubMed 37443319
- Frontiers in cellular neuroscience 17:11629662023Possible involvement of microglial P2RY12 and peripheral IL-10 in postpartum depression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui-Ju Kim,Min-Jung You,Soyoung Sung,Chan Rim,Min-Soo KwonPubMed 37396924
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e229702023Indispensable role of Nectin-like 4 in regulating synapse-related molecules, synaptic structure, and individual behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Liu,Kunnian Ran,Geng Hu,Bin Yin,Boqin Qiang,Wei Han,Pengcheng Shu,Xiaozhong PengPubMed 37184041
- Experimental neurobiology 32:68-822023A Critical Involvement of Glutamatergic Neurons in the Anterior Insular Cortex for Subdiaphragmatic Vagotomy-induced Analgesia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYea Jin Kim,Grace J Lee,Sang Wook Shim,Doyun Kim,Seog Bae OhPubMed 37164647
- British journal of pharmacology 180:1379-14072023Histamine bidirectionally regulates the intrinsic excitability of parvalbumin-positive neurons in the lateral globus pallidus and promotes motor behaviour.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeng-Xin Qi,Kang-Li Shen,Jian-Ya Peng,Xiu-Juan Fan,Hui-Wei Huang,Jian-Lan Jiang,Jian-Hua Lu,Xiao-Qin Wang,Xiao-Xia Fang,Liang Chen,Qian-Xing ZhuangPubMed 36512485
- International journal of molecular sciences 23:2022Enhanced BDNF and TrkB Activation Enhance GABA Neurotransmission in Cerebellum in Hyperammonemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaiza M Arenas,Mar Martínez-García,Marta Llansola,Vicente FelipoPubMed 36233065
- Journal of neurology 270:57-702022Homeostatic plasticity of eye movement performance in Xenopus tadpoles following prolonged visual image motion stimulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Forsthofer,Hans StrakaPubMed 35947153
- Molecular brain 15:532022Downregulation of CDK5 signaling in the dorsal striatum alters striatal microcircuits implicating the association of pathologies with circadian behavior in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHu Zhou,Jingxin Zhang,Huaxiang Shi,Pengfei Li,Xin Sui,Yongan Wang,Liyun WangPubMed 35701839
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human GAD1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q99259
Abcam Recommends
Reactivity data
Select an application| WB | IHC-P | Flow Cyt | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected |
| Mouse | Tested | Tested | Expected | Expected |
| Rat | Expected | Expected | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100000 | Notes - |
Species Mouse | Dilution info 1/5000 - 1/100000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes An antigen retrieval step is necessary (e.g. incubating the sections at 90 degrees C in a 50mM citrate buffer). Cell body labeling is optimized when Triton is omitted from the tissue processing. Axon terminal labeling is substantially increased (and cell body labeling decreased) when Triton is included (see Soghomonian et al, 1998). Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg for 106 Cells | Notes (methanol fixed cells) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor. Isoform 3. Enzymatically inactive as glutamate decarboxylase.
Alternative names
GAD, GAD67, GAD1, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD-67
Recommended products
Mouse monoclonal [K-87] to GAD67/GAD1 is a mouse monoclonal antibody that is used to detect GAD67/GAD1 in Flow cytometry, ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 100 publications
- Trusted since 2005
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human GAD1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q99259
- Clone number
- K-87
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab26116 mouse monoclonal [K-87] to GAD67 specically recognizes GAD67 and has no cross reactivity with GAD65; this has been shown on western blots of mouse brain or purified recombinant GAD67 and GAD65. In immunohistochemical analysis of brain sections, the K-87 monoclonal recognizes GAD67 in nerve cell bodies and has an enhanced ability to detect GAD67 in dendrites and axon buttons compared to the original anti-GAD67 K-2 polyclonal antibody (see Kaufman et al, 1991).
Product SpecificationsMouse monoclonal [K-87] to GAD67/GAD1 (ab26116) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-P, WB in human, mouse, rat samples.
Mouse monoclonal [K-87] to GAD67/GAD1 (ab26116) specifically detects GAD67/GAD1 (UniProt ID: Q99259; Molecular weight: 67kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Mouse monoclonal [K-87] to GAD67/GAD1 (ab26116) has high sensitivity and specificity.
Mouse monoclonal [K-87] to GAD67/GAD1 (ab26116) has been cited over 104 times in peer reviewed journals and is trusted by the scientific community.
Mouse monoclonal [K-87] to GAD67/GAD1 (ab26116) has 19 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--western-blot-img153993.jpg "Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116)")
Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116)
GAD67/GAD1 Western blot staining of mouse cerebellum using mouse Anti-GAD67/GAD1 antibody
All lanes: Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116) at 1/2000 dilution
All lanes: mouse cerebellum at 0.75 µg
SecondaryAll lanes: HRP horse anti-mouse IgG at 1/5000 dilution
Predicted band size: 66 kDa
![Flow Cytometry - Anti-GAD67/GAD1 antibody [K-87] (ab26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--flow-cytometry-img50857.jpg "Flow Cytometry - Anti-GAD67/GAD1 antibody [K-87] (ab26116)")
Flow Cytometry - Anti-GAD67/GAD1 antibody [K-87] (ab26116)
GAD67/GAD1 Flow Cytometry staining using mouse Anti-GAD67/GAD1 antibody
Overlay histogram showing PC12 cells stained with ab26116 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (ab26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110186.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (ab26116)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (ab26116)
GAD67/GAD1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-GAD67/GAD1 antibody
ab26116, at a 1/2000 dilution, staining of fixed mouse cerebellum sections that underwent an antigen retrieval step.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (ab26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110010.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (ab26116)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (ab26116)
GAD67/GAD1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-GAD67/GAD1 antibody
Immunohistochemical staining of mouse pancreatic islet beta cells after antigen retrieval with ab26116 at a dilution of 1/1000.
![Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody [K-87] (ab26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--immunocytochemistry-immunofluorescence-img104499.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody [K-87] (ab26116)")
Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody [K-87] (ab26116)
GAD67/GAD1 Immunocytochemistry/ Immunofluorescence staining using mouse Anti-GAD67/GAD1 antibody
ICC/IF image of ab26116 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 10μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
![Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--western-blot-img428907.jpg "Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116)")
Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116)
GAD67/GAD1 Western blot staining of Human brain using mouse Anti-GAD67/GAD1 antibody
All lanes: Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116) at 1/100000 dilution
All lanes: Human brain
SecondaryAll lanes: Rabbit anti-mouse antibody
![Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116), expandable thumbnail](https://content.abcam.com/products/images/gad67-gad1-antibody-k-87-ab26116--western-blot-img428907.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com