Anti-GAD67/GAD1 antibody [K-87]

• WB

Supplier Data

Western blot - Anti-GAD67/GAD1 antibody [K-87] (AB26116)

All lanes:

Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116) at 1/2000 dilution

All lanes:

mouse cerebellum at 0.75 µg

Secondary

All lanes:

HRP horse anti-mouse IgG at 1/5000 dilution

Predicted band size: 66 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (AB26116)

ab26116, at a 1/2000 dilution, staining of fixed mouse cerebellum sections that underwent an antigen retrieval step.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GAD67/GAD1 antibody [K-87] (AB26116)

ICC/IF image of ab26116 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 10μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• Flow Cyt

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Flow Cytometry - Anti-GAD67/GAD1 antibody [K-87] (AB26116)

Overlay histogram showing PC12 cells stained with ab26116 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD67/GAD1 antibody [K-87] (AB26116)

Immunohistochemical staining of mouse pancreatic islet beta cells after antigen retrieval with ab26116 at a dilution of 1/1000.

• WB

Supplier Data

Western blot - Anti-GAD67/GAD1 antibody [K-87] (AB26116)

All lanes:

Western blot - Anti-GAD67/GAD1 antibody [K-87] (ab26116) at 1/100000 dilution

All lanes:

Human brain

Secondary

All lanes:

Rabbit anti-mouse antibody

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