AB175355

Anti-GADD34 antibody

Name: Anti-GADD34 antibody (ab175355) | Abcam
Brand: Abcam Limited
SKU: AB175355
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GADD34 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R15A aa 550-650.

View Alternative Names

GADD34, PPP1R15A, Protein phosphatase 1 regulatory subunit 15A, Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody (AB175355)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody (AB175355)

Immunofluorescent analysis of formalin-fixed HeLa cells labeling GADD34 with ab175355 at 1/50 and incubated with DyLight 488 Goat anti-rabbit IgG secondary antibody. F-actin (red) was stained with DyLight 650 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

Supplier Data

Western blot - Anti-GADD34 antibody (AB175355)

Lane 2:

Western blot - Anti-GADD34 antibody (ab175355) at 1/250 dilution

Lanes 3 and 5:

Western blot - Anti-GADD34 antibody (ab175355) at 1/500 dilution

Lane 4:

Western blot - Anti-GADD34 antibody (ab175355) at 1/1000 dilution

Lanes 2 - 4:

HeLa cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg with neutralizing peptide

Secondary

All lanes:

Goat anti-rabbit IgG-HRP secondary antibody

Predicted band size: 73 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PPP1R15A aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

O75807

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress (PubMed : 26095357, PubMed : 26742780). Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1 (PubMed : 14718519). May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15' (PubMed : 14635196). Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux (PubMed : 32978159). Also acts a viral restriction factor by attenuating HIV-1 replication (PubMed : 31778897). Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation (PubMed : 31778897).. (Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR.

See full target information PPP1R15A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:13575-13592 PubMed41054626

2025

Sirp-α Antibody Inhibits Renal Cell Carcinoma Progression via Akt1/Akt2 Modulation in Tumor-Associated Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Hao,Naiquan Liu,Xin Huang,Hanlei Zhou,Hanrong Li,Yizhou Zhang,Bing Yu,Ziqian Bi,Xinyuan Song,Shunan Li,Keyu Chen,Ning Li,Chao Zhu,Jiahe Wang

Cell death & disease 13:425 PubMed35501353

2022

Mxi1 participates in the progression of lung cancer via the microRNA-300/KLF9/GADD34 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Lei,Yunchao Huang,Jianbin Lin,Shihui Sun,Keda Che,Junting Shen,Jun Liao,Yangming Chen,Kai Chen,Zhaoxian Lin,Xing Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GADD34 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody (AB175355)

Western blot - Anti-GADD34 antibody (AB175355)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise