Anti-GADD34 antibody

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (AB9869)

IHC image of ab9869 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9869, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (AB9869)

ab9869 staining GADD34 in human liver sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• WB

Supplier Data

Western blot - Anti-GADD34 antibody (AB9869)

Primary incubation for 1 hour. Detection by chemiluminescence.

All lanes:

Western blot - Anti-GADD34 antibody (ab9869) at 0.1 µg/mL

All lanes:

HEPG2 cell lysate at 35 µg

Predicted band size: 73 kDa

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