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AB186120

Anti-GAK antibody

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(2 Publications)

Rabbit Polyclonal GAK antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GAK aa 650-750.

View Alternative Names

DNAJC26, GAK, Cyclin-G-associated kinase, DnaJ homolog subfamily C member 26

2 Images
Western blot - Anti-GAK antibody (AB186120)
  • WB

Supplier Data

Western blot - Anti-GAK antibody (AB186120)

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-GAK antibody (ab186120) at 1/1000 dilution

Lane 1:

GaMG Whole cell lysate at 10 µg

Lane 2:

HeLa Whole cell lysate at 10 µg

Lane 3:

HEK293T Whole cell lysate at 10 µg

Lane 4:

Jurkat Whole cell lysate at 10 µg

Lane 5:

RKO Whole cell lysate at 10 µg

Predicted band size: 143 kDa

false

Western blot - Anti-GAK antibody (AB186120)
  • WB

Supplier Data

Western blot - Anti-GAK antibody (AB186120)

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Western blot - Anti-GAK antibody (ab186120) at 1/1000 dilution

Lane 1:

CT26 Whole cell lysate at 10 µg

Lane 2:

CH27 Whole cell lysate at 10 µg

Lane 3:

TCMK-1 Whole cell lysate at 10 µg

Predicted band size: 143 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human GAK aa 650-750. The exact immunogen used to generate this antibody is proprietary information.

O14976

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (PubMed : 10625686). May play a role in clathrin-mediated endocytosis and intracellular trafficking, and in the dynamics of clathrin assembly/disassembly (PubMed : 18489706).
See full target information GAK
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Antiviral research 204:105367 PubMed35738348

2022

Numb-associated kinases are required for SARS-CoV-2 infection and are cellular targets for antiviral strategies.

Applications

Unspecified application

Species

Unspecified reactive species

Marwah Karim,Sirle Saul,Luca Ghita,Malaya Kumar Sahoo,Chengjin Ye,Nishank Bhalla,Chieh-Wen Lo,Jing Jin,Jun-Gyu Park,Belén Martinez-Gualda,Michael Patrick East,Gary L Johnson,Benjamin A Pinsky,Luis Martinez-Sobrido,Christopher R M Asquith,Aarthi Narayanan,Steven De Jonghe,Shirit Einav

Molecules (Basel, Switzerland) 25: PubMed32570757

2020

FAM83G Is a Novel Inducer of Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junichi Okada,Noriaki Sunaga,Eijiro Yamada,Tsugumichi Saito,Atsushi Ozawa,Yasuyo Nakajima,Kazuya Okada,Jeffrey E Pessin,Shuichi Okada,Masanobu Yamada
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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