Anti-GAK antibody [EPR25327-39] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAK antibody [EPR25327-39] - BSA and Azide free (AB305070)

This data was developed using ab305101, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling GAK with ab305101 at 1/50 (9.9 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on human testis (PMID : 9299234). The section was incubated with ab305101 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAK antibody [EPR25327-39] - BSA and Azide free (AB305070)

This data was developed using ab305101, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GAK with ab305101 at 1/50 (9.9 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on human breast carcinoma (PMID : 16161052). The section was incubated with ab305101 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAK antibody [EPR25327-39] - BSA and Azide free (AB305070)

This data was developed using ab305101, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK293 tissue labeling GAK with ab305101 at 1/50 (9.9 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on (A) Wild-type HEK293T cell pellet, no staining on (B) GAK knockout HEK293T cell pellet. The section was incubated with ab305101 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• WB

Supplier Data

Western blot - Anti-GAK antibody [EPR25327-39] - BSA and Azide free (AB305070)

This data was developed using ab305101, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 92 seconds

All lanes:

Western blot - Anti-GAK antibody [EPR25327-39] (<a href='/en-us/products/primary-antibodies/gak-antibody-epr25327-39-ab305101'>ab305101</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 160 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-GAK antibody [EPR25327-39] - BSA and Azide free (AB305070)

This data was developed using ab305101, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, ab305101 was shown to bind specifically to GAK. A band was observed at 160 kDa in wild-type 293T cell lysates with no signal observed at this size in GAK knockout cell line ab266417 (knockout cell lysate ab263210). Exposure time : 48 seconds

All lanes:

Western blot - Anti-GAK antibody [EPR25327-39] (<a href='/en-us/products/primary-antibodies/gak-antibody-epr25327-39-ab305101'>ab305101</a>) at 1/1000 dilution

Lane 1:

Wild-type 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

GAK knockout HeLa whole cell lysate at 20 µg

Lane 3:

Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate at 20 µg

Lane 4:

Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 160 kDa

false

Exposure time: 48s