AB135398

Anti-GAL4 antibody [14-7E10G10]

Name: Anti-GAL4 antibody [14-7E10G10] (ab135398) | Abcam
Brand: Abcam Limited
SKU: AB135398
Price: 530 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal GAL4 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications.

View Alternative Names

YPL248C, GAL4, Regulatory protein GAL4

1 Images

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Western blot - Anti-GAL4 antibody [14-7E10G10] (AB135398)

All lanes:

Western blot - Anti-GAL4 antibody [14-7E10G10] (ab135398) at 0.5 µg/mL

Lane 1:

Whole cell yeast lysate control from untransformed SEY6210 pep4-3 strain (a kind gift from Prof. Tom Stevens, University of Oregon).

Lane 2:

Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJK22-pGBDU plasmid containing GAL4 DNA Binding domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).

Lane 3:

Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJk23-pGAD plasmid containing GAL4 Active Domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).

Secondary

Lane 1:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) - 1:10000

Lane 2:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP)- 1:10000

Lane 3:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) - 1:10000

Predicted band size: 99 kDa

Observed band size: 56 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

14-7E10G10

Isotype

IgG2b

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

The GAL4 Activation Domain monoclonal antibody binds specifically to the AD of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of fusion proteins containing GAL4 AD as the fusion partner in two hybrid studies. The activation domain is typically used in a two-hybrid experiment as the fusion partner for a library of cDNA clones to screen against a protein of interest fused to a DNA-binding domain. Gal4is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GAL4 also known as a transcription factor is an important protein in yeast. It plays a critical role in regulating the transcription of genes involved in galactose metabolism. The GAL4 protein has a mass of approximately 881 amino acids and is mainly expressed in the nucleus of yeast cells. Common parts of GAL4 include the GAL4 DBD (DNA-binding domain) and GAL4 AD (activation domain) which are critical for its function. Although similar sounding GAL4 should not be confused with galectin-4 or galectin 4 which are different proteins.

Biological function summary

The GAL4 transcription factor functions as a major regulator of galactose-responsive genes. It acts as part of a complex binding with its DNA target sites to initiate transcription of GAL genes like GAL1 GAL7 and GAL10. GAL4 protein recruits transcriptional machinery through its activation domain working alongside other factors to turn on specific gene expression in response to the presence of galactose. This capacity for gene regulation underlines its essential role in sugar metabolism in yeast.

Pathways

GAL4 is intimately involved in the GAL gene regulatory pathway that manages the cellular response to galactose. In this pathway proteins such as GAL80 inhibit GAL4 function under non-inducing conditions. However in the presence of galactose GAL3 binds and neutralizes GAL80 liberating GAL4 to activate gene expression. This system exemplifies a dynamic interaction in yeast allowing precise gene regulation in accordance with sugar availability.

While GAL4 is specifically a yeast protein and does not directly relate to human diseases its functional analogs and concepts have implications in genetic research and synthetic biology. For example GAL4 system is widely used in Drosophila and other model organisms to study gene expression patterns and related disorders. This system compared with mammalian transcription factors provides a foundational methodology for exploring genetic regulation mechanisms and potential implications in understanding metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.

See full target information GAL4

Additional targets

GAL4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

mSystems 9:e0100424 PubMed39526800

2024

A comprehensive two-hybrid analysis to explore the effector-effector interactome.

Applications

Unspecified application

Species

Unspecified reactive species

Harley O'Connor Mount,Malene L Urbanus,Dayag Sheykhkarimli,Atina G Coté,Florent Laval,Georges Coppin,Nishka Kishore,Roujia Li,Kerstin Spirohn-Fitzgerald,Morgan O Petersen,Jennifer J Knapp,Dae-Kyum Kim,Jean-Claude Twizere,Michael A Calderwood,Marc Vidal,Frederick P Roth,Alexander W Ensminger

PLoS pathogens 20:e1012662 PubMed39514589

2024

Use of the Puccinia sorghi haustorial transcriptome to identify and characterize AvrRp1-D recognized by the maize Rp1-D resistance protein.

Applications

Unspecified application

Species

Unspecified reactive species

Saet-Byul Kim,Ki-Tae Kim,Solhee In,Namrata Jaiswal,Gir-Won Lee,Seungmee Jung,Abigail Rogers,Libia F Gómez-Trejo,Sujan Gautam,Matthew Helm,Hee-Kyung Ahn,Hye-Young Lee,Quentin D Read,Jongchan Woo,Katerina L Holan,Steven A Whitham,Jonathan D G Jones,Doil Choi,Ralph Dean,Eunsook Park,Peter Balint-Kurti

mBio 15:e0322123 PubMed38335095

2024

An acetyltransferase effector conserved across species targets the eukaryotic eIF3 complex to modulate protein translation.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Syriste,Deepak T Patel,Peter J Stogios,Tatiana Skarina,Dhruvin Patel,Alexei Savchenko

View all publications

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Product promise

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Anti-GAL4 antibody [14-7E10G10]

Western blot - Anti-GAL4 antibody [14-7E10G10] (AB135398)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

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