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AB135398

Anti-GAL4 antibody [14-7E10G10]

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(3 Publications)

Mouse Monoclonal GAL4 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications.

View Alternative Names

YPL248C, GAL4, Regulatory protein GAL4, GAL81, Gal4p, Regulatory protein GAL4

1 Images
Western blot - Anti-GAL4 antibody [14-7E10G10] (AB135398)
  • WB

Unknown

Western blot - Anti-GAL4 antibody [14-7E10G10] (AB135398)

All lanes:

Western blot - Anti-GAL4 antibody [14-7E10G10] (ab135398) at 0.5 µg/mL

Lane 1:

Whole cell yeast lysate control from untransformed SEY6210 pep4-3 strain (a kind gift from Prof. Tom Stevens, University of Oregon).

Lane 2:

Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJK22-pGBDU plasmid containing GAL4 DNA Binding domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).

Lane 3:

Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJk23-pGAD plasmid containing GAL4 Active Domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).

Secondary

Lane 1:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) - 1:10000

Lane 2:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP)- 1:10000

Lane 3:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) - 1:10000

Predicted band size: 99 kDa

Observed band size: 56 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

14-7E10G10

Isotype

IgG2b

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

The GAL4 Activation Domain monoclonal antibody binds specifically to the AD of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of fusion proteins containing GAL4 AD as the fusion partner in two hybrid studies. The activation domain is typically used in a two-hybrid experiment as the fusion partner for a library of cDNA clones to screen against a protein of interest fused to a DNA-binding domain. Gal4is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Precipitation Ammonium Sulphate
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
See full target information GAL4

Additional targets

GAL4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

mSystems 9:e0100424 PubMed39526800

2024

A comprehensive two-hybrid analysis to explore the effector-effector interactome.

Applications

Unspecified application

Species

Unspecified reactive species

Harley O'Connor Mount,Malene L Urbanus,Dayag Sheykhkarimli,Atina G Coté,Florent Laval,Georges Coppin,Nishka Kishore,Roujia Li,Kerstin Spirohn-Fitzgerald,Morgan O Petersen,Jennifer J Knapp,Dae-Kyum Kim,Jean-Claude Twizere,Michael A Calderwood,Marc Vidal,Frederick P Roth,Alexander W Ensminger

PLoS pathogens 20:e1012662 PubMed39514589

2024

Use of the Puccinia sorghi haustorial transcriptome to identify and characterize AvrRp1-D recognized by the maize Rp1-D resistance protein.

Applications

Unspecified application

Species

Unspecified reactive species

Saet-Byul Kim,Ki-Tae Kim,Solhee In,Namrata Jaiswal,Gir-Won Lee,Seungmee Jung,Abigail Rogers,Libia F Gómez-Trejo,Sujan Gautam,Matthew Helm,Hee-Kyung Ahn,Hye-Young Lee,Quentin D Read,Jongchan Woo,Katerina L Holan,Steven A Whitham,Jonathan D G Jones,Doil Choi,Ralph Dean,Eunsook Park,Peter Balint-Kurti

mBio 15:e0322123 PubMed38335095

2024

An acetyltransferase effector conserved across species targets the eukaryotic eIF3 complex to modulate protein translation.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Syriste,Deepak T Patel,Peter J Stogios,Tatiana Skarina,Dhruvin Patel,Alexei Savchenko
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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