Mouse Monoclonal GAL4 antibody. Suitable for WB and reacts with Transfected cell lysate - Saccharomyces cerevisiae samples. Cited in 4 publications.
Images
![Western blot - Anti-GAL4 antibody [15-6E10A7] (AB135397), expandable thumbnail](https://content.abcam.com/products/images/gal4-antibody-15-6e10a7-ab135397--western-blot-img7613.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAL4 antibody [15-6E10A7] (AB135397)")
Publications
Filter by
- Genome biology 23:502022An improved repertoire of splicing variants and their potential roles in Arabidopsis photomorphogenic development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Kai Huang,Wen-Dar Lin,Shu-Hsing WuPubMed 35139889
- Molecular cell 74:996-1009.e72019RRM Transcription Factors Interact with NLRs and Regulate Broad-Spectrum Blast Resistance in Rice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeran Zhai et. al.PubMed 30975460
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 30:174-852015NF-κB-repressing factor phosphorylation regulates transcription elongation via its interactions with 5'→3' exoribonuclease 2 and negative elongation factor.Applications:WBReactive species:Unspecified reactive speciesSascha Rother,Myriam Bartels,Aike Torben Schweda,Klaus Resch,Norbert Pallua,Mahtab NourbakhshPubMed 26340924
- The Journal of biological chemistry 290:20661-732015Protein Interaction between Ameloblastin and Proteasome Subunit α Type 3 Can Facilitate Redistribution of Ameloblastin Domains within Forming Enamel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuhui Geng et. al.PubMed 26070558
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Saccharomyces cerevisiae | Predicted |
| Transfected cell lysate - Saccharomyces cerevisiae | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Saccharomyces cerevisiae | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
Additional Targets
GAL4
Alternative names
YPL248C, GAL4, Regulatory protein GAL4
Recommended products
Mouse Monoclonal GAL4 antibody. Suitable for WB and reacts with Transfected cell lysate - Saccharomyces cerevisiae samples. Cited in 4 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 15-6E10A7
- Purification technique
- Precipitation Ammonium Sulphate
- Specificity
This antibody binds specifically to the DNA binding domain of the yeast GAL4 protein.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
The GAL4 DNA-BD monoclonal antibody binds specifically to the DNA-binding domain of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of bait fusion proteins containing GAL4 DNA BD as the fusion partner in yeast two hybrid studies. The binding domain is typically used in a two-hybrid experiment as the fusion partner for the protein of interest. When the fusion protein of interest cannot activate transcription on its own, it can be used as bait to screen a library of cDNA clones that are fused to an activation domain. GAL4 is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GAL4 also known as a transcription factor is an important protein in yeast. It plays a critical role in regulating the transcription of genes involved in galactose metabolism. The GAL4 protein has a mass of approximately 881 amino acids and is mainly expressed in the nucleus of yeast cells. Common parts of GAL4 include the GAL4 DBD (DNA-binding domain) and GAL4 AD (activation domain) which are critical for its function. Although similar sounding GAL4 should not be confused with galectin-4 or galectin 4 which are different proteins.
- Biological function summary
The GAL4 transcription factor functions as a major regulator of galactose-responsive genes. It acts as part of a complex binding with its DNA target sites to initiate transcription of GAL genes like GAL1 GAL7 and GAL10. GAL4 protein recruits transcriptional machinery through its activation domain working alongside other factors to turn on specific gene expression in response to the presence of galactose. This capacity for gene regulation underlines its essential role in sugar metabolism in yeast.
- Pathways
GAL4 is intimately involved in the GAL gene regulatory pathway that manages the cellular response to galactose. In this pathway proteins such as GAL80 inhibit GAL4 function under non-inducing conditions. However in the presence of galactose GAL3 binds and neutralizes GAL80 liberating GAL4 to activate gene expression. This system exemplifies a dynamic interaction in yeast allowing precise gene regulation in accordance with sugar availability.
- Associated diseases and disorders
While GAL4 is specifically a yeast protein and does not directly relate to human diseases its functional analogs and concepts have implications in genetic research and synthetic biology. For example GAL4 system is widely used in Drosophila and other model organisms to study gene expression patterns and related disorders. This system compared with mammalian transcription factors provides a foundational methodology for exploring genetic regulation mechanisms and potential implications in understanding metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-GAL4 antibody [15-6E10A7] (ab135397), expandable thumbnail](https://content.abcam.com/products/images/gal4-antibody-15-6e10a7-ab135397--western-blot-img7613.jpg "Western blot - Anti-GAL4 antibody [15-6E10A7] (ab135397)")
Western blot - Anti-GAL4 antibody [15-6E10A7] (ab135397)
All lanes: Western blot - Anti-GAL4 antibody [15-6E10A7] (ab135397) at 1 µg/mL
Lane 1: Whole cell yeast lysate control from untransformed SEY6210 pep4-3 strain (a kind gift from Prof. Tom Stevens, University of Oregon).
Lane 2: Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJK22-pGBDU plasmid containing GAL4 DNA Binding domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).
Lane 3: Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJk23-pGAD plasmid containing GAL4 Active Domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).
SecondaryAll lanes: Anti-Mouse IgG (H&L) Goat Polyclonal
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 56 kDa
Exposure time: 3min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com