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AB175185

Anti-GAL4 antibody [EPR12710(B)]

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(3 Publications )

Rabbit Recombinant Monoclonal GAL4 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 3 publications.

View Alternative Names

Galectin-4, Gal-4, Antigen NY-CO-27, L-36 lactose-binding protein, Lactose-binding lectin 4, L36LBP, LGALS4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (AB175185)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (AB175185)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GAL4 with ab175185 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (AB175185)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (AB175185)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling GAL4 with ab175185 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-GAL4 antibody [EPR12710(B)] (AB175185)
  • WB

Supplier Data

Western blot - Anti-GAL4 antibody [EPR12710(B)] (AB175185)

All lanes:

Western blot - Anti-GAL4 antibody [EPR12710(B)] (ab175185) at 1/1000 dilution

Lane 1:

HT-29 cell lysates at 10 µg

Lane 2:

Human small intestine lysates at 10 µg

Lane 3:

Rat small intestine lysates at 10 µg

Predicted band size: 36 kDa

false

  • Carrier free

    Anti-GAL4 antibody [EPR12710(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12710(B)

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
See full target information Galectin-4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering & translational medicine 10:e70021 PubMed41030277

2025

Engineered lung cell targeting and SLC7A11 siRNA expressing bacterial extracellular vesicles impair the progression of none-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Dan Wan,Xue-Liang Zhou,Jin-Long Liu,Hua Xu

Nature communications 13:7578 PubMed36481721

2022

Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Chen,Chunyu Yu,Xinhua Liu,Beibei Liu,Xiaodi Wu,Jiajing Wu,Dong Yan,Lulu Han,Zifan Tang,Xinyi Yuan,Jianqiu Wang,Yue Wang,Shumeng Liu,Lin Shan,Yongfeng Shang

The European journal of neuroscience 44:2226-35 PubMed27306299

2016

Brain BDNF levels are dependent on cerebrovascular endothelium-derived nitric oxide.

Applications

Unspecified application

Species

Unspecified reactive species

Hayat Banoujaafar,Alice Monnier,Nicolas Pernet,Aurore Quirié,Philippe Garnier,Anne Prigent-Tessier,Christine Marie
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com