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AB230924

Anti-Galactosidase alpha antibody

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(1 Publication)

Rabbit Polyclonal Galactosidase alpha antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GLA aa 150 to C-terminus.

View Alternative Names

Alpha-galactosidase A, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Galactosylgalactosylglucosylceramidase GLA, Melibiase, GLA

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Galactosidase alpha antibody (AB230924)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Galactosidase alpha antibody (AB230924)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Galactosidase alpha (green) using ab230924 at 1/50 dilution in ICC/IF.

The nuclear counter stain is DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody (AB230924)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody (AB230924)

Formalin-fixed, paraffin-embedded human adrenal tissue stained for Galactosidase alpha with ab230924 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Galactosidase alpha antibody (AB230924)
  • WB

Supplier Data

Western blot - Anti-Galactosidase alpha antibody (AB230924)

All lanes:

Western blot - Anti-Galactosidase alpha antibody (ab230924) at 1/500 dilution

Lane 1:

SW620 cell extract

Lane 2:

BT-474 (human ductal breast epithelial tumor cell line) cell extract

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GLA aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P06280

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-galactosidase also known as alpha-D-galactosidase or A-galactosidase is an enzyme that catalyzes the hydrolysis of terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It has a molecular mass of approximately 50000 Daltons. This enzyme is expressed in various tissues including the liver heart and kidneys. It functions by efficiently removing alpha-galactose residues from target molecules a process important for maintaining cellular function.
Biological function summary

The role of alpha-galactosidase involves the breakdown of complex carbohydrates particularly those containing alpha-galactosidic bonds. It does not operate as part of a complex but rather functions independently to cleave these specific bonds in glycolipids and glycoproteins. This activity is critical for degrading langerin and other gangliosides preventing the accumulation of these molecules in the lysosomes. By continuously performing this function alpha-galactosidase maintains glycosphingolipid homeostasis ensuring efficient cellular metabolism.

Pathways

The involvement of alpha-galactosidase in metabolic and lysosomal catabolic pathways is significant. One critical pathway is the glycolipid metabolism pathway where it acts alongside related enzymes such as beta-galactosidase. It also participates in the lysosomal degradation pathway working in conjunction with other lysosomal enzymes to prevent substrate buildup. The interaction with enzymes like lysosomal hydrolases ensures the proper breakdown of complex molecules into simpler ones that cells can utilize or excrete.

Defects in the alpha-galactosidase enzyme lead to the lysosomal storage disorder known as Fabry disease. This genetic disorder causes the accumulation of globotriaosylceramide due to deficient alpha-galactosidase activity. Another disorder associated with malfunctioning alpha-galactosidase is cardiac complications due to tissue storage of glycolipids. In both cases the lack of enzyme activity disrupts cellular and tissue functions linking alpha-galactosidase to clinical symptoms seen in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of glycosphingolipids and participates in their degradation in the lysosome.
See full target information GLA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental hematology & oncology 12:4 PubMed36624493

2023

Alpha-B-Crystallin overexpression is sufficient to promote tumorigenesis and metastasis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Behnam Rashidieh,Amanda Louise Bain,Simon Manuel Tria,Sowmya Sharma,Cameron Allan Stewart,Jacinta Ley Simmons,Pirjo M Apaja,Pascal H G Duijf,John Finnie,Kum Kum Khanna
View all publications
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Product promise

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