Rabbit Recombinant Monoclonal Galactosidase alpha antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (AB215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--western-blot-img10441.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (AB215258)")
![Immunocytochemistry/ Immunofluorescence - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (AB215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--immunocytochemistry-immunofluorescence-img14281.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (AB215258)")
![Immunoprecipitation - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (AB215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--immunoprecipitation-img9243.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (AB215258)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (AB215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114976.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (AB215258)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (AB215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91191.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (AB215258)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
3 products for Alternative Product
4 products for Alternative Version
Target data
Function
Catalyzes the hydrolysis of glycosphingolipids and participates in their degradation in the lysosome.
Alternative names
Alpha-galactosidase A, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Galactosylgalactosylglucosylceramidase GLA, Melibiase, GLA
Recommended products
Rabbit Recombinant Monoclonal Galactosidase alpha antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP5828(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab215258 is the carrier-free version of Anti-Galactosidase alpha antibody [EP5828(2)] ab168341.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-galactosidase also known as alpha-D-galactosidase or A-galactosidase is an enzyme that catalyzes the hydrolysis of terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It has a molecular mass of approximately 50000 Daltons. This enzyme is expressed in various tissues including the liver heart and kidneys. It functions by efficiently removing alpha-galactose residues from target molecules a process important for maintaining cellular function.
- Biological function summary
The role of alpha-galactosidase involves the breakdown of complex carbohydrates particularly those containing alpha-galactosidic bonds. It does not operate as part of a complex but rather functions independently to cleave these specific bonds in glycolipids and glycoproteins. This activity is critical for degrading langerin and other gangliosides preventing the accumulation of these molecules in the lysosomes. By continuously performing this function alpha-galactosidase maintains glycosphingolipid homeostasis ensuring efficient cellular metabolism.
- Pathways
The involvement of alpha-galactosidase in metabolic and lysosomal catabolic pathways is significant. One critical pathway is the glycolipid metabolism pathway where it acts alongside related enzymes such as beta-galactosidase. It also participates in the lysosomal degradation pathway working in conjunction with other lysosomal enzymes to prevent substrate buildup. The interaction with enzymes like lysosomal hydrolases ensures the proper breakdown of complex molecules into simpler ones that cells can utilize or excrete.
- Associated diseases and disorders
Defects in the alpha-galactosidase enzyme lead to the lysosomal storage disorder known as Fabry disease. This genetic disorder causes the accumulation of globotriaosylceramide due to deficient alpha-galactosidase activity. Another disorder associated with malfunctioning alpha-galactosidase is cardiac complications due to tissue storage of glycolipids. In both cases the lack of enzyme activity disrupts cellular and tissue functions linking alpha-galactosidase to clinical symptoms seen in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--western-blot-img10441.jpg "Western blot - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)")
Western blot - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Galactosidase alpha antibody [EP5828(2)] ab168341).
Lanes 1-2: Merged signal (red and green). Green - Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 observed at 49 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 Anti-Galactosidase alpha antibody [EP5828(2)] was shown to specifically react with Galactosidase alpha in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human GLA (Galactosidase alpha) knockout HeLa cell line ab265563 (knockout cell lysate Human GLA (Galactosidase alpha) knockout HeLa cell lysate ab257449) was used. Wild-type and Galactosidase alpha knockout samples were subjected to SDS-PAGE. Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Galactosidase alpha antibody [EP5828(2)] (Anti-Galactosidase alpha antibody [EP5828(2)] ab168341) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: GLA knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human GLA (Galactosidase alpha) knockout HeLa cell line (Human GLA (Galactosidase alpha) knockout HeLa cell line ab265563)
Performed under reducing conditions.
Predicted band size: 38 kDa, 49 kDa
Observed band size: 49 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--immunocytochemistry-immunofluorescence-img14281.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)")
Immunocytochemistry/ Immunofluorescence - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Galactosidase with purified Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/500) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galactosidase alpha antibody [EP5828(2)] ab168341).
![Immunoprecipitation - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--immunoprecipitation-img9243.jpg "Immunoprecipitation - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)")
Immunoprecipitation - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)
Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 (purified) at 1/60 immunoprecipitating Galactosidase alpha in MCF-7 whole cell lysate.
Lane 1 (input): MCF-7 whole cell lysate (10µg)
Lane 2 (+): Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 + MCF-7 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galactosidase alpha antibody [EP5828(2)] ab168341).
All lanes: Immunoprecipitation - Anti-Galactosidase alpha antibody [EP5828(2)] (Anti-Galactosidase alpha antibody [EP5828(2)] ab168341)
Predicted band size: 49 kDa
Observed band size: 46 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114976.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling Galactosidase alpha with unpurified Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 at a dilution of 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galactosidase alpha antibody [EP5828(2)] ab168341).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91191.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Galactosidase alpha with purified Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galactosidase alpha antibody [EP5828(2)] ab168341).
![Flow Cytometry (Intracellular) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--flow-cytometry-intracellular-img25782.jpg "Flow Cytometry (Intracellular) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)")
Flow Cytometry (Intracellular) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)
Intracellular Flow Cytometry analysis of HeLa cells labelling Galactosidase alpha with purified Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 at a dilution of 1/90 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galactosidase alpha antibody [EP5828(2)] ab168341).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78187.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human urinary bladder carcinoma tissue labelling Galactosidase alpha with unpurified Anti-Galactosidase alpha antibody [EP5828(2)] ab168341 at a dilution of 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galactosidase alpha antibody [EP5828(2)] ab168341).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--flow-cytometry-intracellular-img25782.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258), expandable thumbnail](https://content.abcam.com/products/images/galactosidase-alpha-antibody-ep58282-bsa-and-azide-free-ab215258--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78187.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactosidase alpha antibody [EP5828(2)] - BSA and Azide free (ab215258)")
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