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AB198712

Anti-Galactoside 2-alpha-L-fucosyltransferase 1 antibody

5

(2 Reviews)

|

(4 Publications)

Rabbit Polyclonal Galactoside 2-alpha-L-fucosyltransferase 1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human FUT1.

View Alternative Names

H, HSC, FUT1, Blood group H alpha 2-fucosyltransferase, Fucosyltransferase 1, GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactoside 2-alpha-L-fucosyltransferase 1 antibody (AB198712)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactoside 2-alpha-L-fucosyltransferase 1 antibody (AB198712)

Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using ab 198712 at dilution 1/35.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactoside 2-alpha-L-fucosyltransferase 1 antibody (AB198712)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galactoside 2-alpha-L-fucosyltransferase 1 antibody (AB198712)

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using ab 198712 at dilution 1/35.

Western blot - Anti-Galactoside 2-alpha-L-fucosyltransferase 1 antibody (AB198712)
  • WB

Unknown

Western blot - Anti-Galactoside 2-alpha-L-fucosyltransferase 1 antibody (AB198712)

Gel : 8%SDS-PAGE

All lanes:

Western blot - Anti-Galactoside 2-alpha-L-fucosyltransferase 1 antibody (ab198712) at 1/800 dilution

Lane 1:

Human cerebella tissue at 40 µg

Lane 2:

Human cerebrum tissue at 40 µg

Lane 3:

Raji cell lysates at 40 µg

Secondary

All lanes:

Goat anti rabbit IgG at 1/5000 dilution

Predicted band size: 41 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human FUT1. The exact immunogen used to generate this antibody is proprietary information.

P19526

Specificity

May cross react with FUT2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Product details

The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of a precursor of the H antigen, which is required for the final step in the soluble A and B antigen synthesis pathway. This gene is one of two encoding the galactoside 2-L-fucosyltransferase enzyme. Mutations in this gene are a cause of the H-Bombay blood group.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galactoside 2-alpha-L-fucosyltransferase 1 also known as FUT1 is an enzyme that plays a role in the addition of fucose a hexose sugar to galactose residues on glycoconjugates. FUT1 has a molecular weight of approximately 44 kDa. It is expressed in various tissues including the liver gastrointestinal tract and endothelial cells indicating a broad functional presence. By transferring fucose in an alpha-12 linkage FUT1 modifies glycoproteins and glycolipids impacting their structure and function.
Biological function summary

FUT1 contributes to the synthesis of H antigen a precursor for ABO blood group antigens by adding fucose to galactoside. It does not usually function as part of a larger protein complex but can have interaction with other components of the glycosylation machinery to ensure proper cellular glycan composition. The action of FUT1 influences cell-cell adhesion signaling and the immune response by shaping cell surface glycan landscapes.

Pathways

FUT1 participates in glycosylation pathways critical for proper cellular recognition and signaling. It plays an important role in the glycosphingolipid biosynthesis pathway that involves the synthesis of the H antigen important for blood group antigen formation. In these pathways FUT1 works in conjunction with other fucosyltransferase family members like FUT2 jointly contributing to the overall diversity of glycan structures found on the cell surface.

FUT1 has implications in several conditions due to its role in glycosylation. Abnormal expression levels can influence the development of certain cancers where altered glycosylation patterns contribute to cell metastasis and immune evasion. Additionally FUT1 modifications could affect the susceptibility to infections by pathogens that recognize specific glycan structures. FUT1's role in cancer is often examined in connection with proteins related to cell adhesion and the immune system such as selectins which interact with glycan structures for cell trafficking and immune response regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to the terminal galactose residue of glycoconjugates through an alpha(1,2) linkage leading to H antigen synthesis that is an intermediate substrate in the synthesis of ABO blood group antigens (PubMed : 2118655). H antigen is essential for maturation of the glomerular layer of the main olfactory bulb, in cell migration and early cell-cell contacts during tumor associated angiogenesis (PubMed : 18205178). Preferentially fucosylates soluble lactose and to a lesser extent fucosylates glycolipids gangliosides GA1 and GM1a (By similarity).
See full target information FUT1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36142211

2022

The Relative Abundances of Human Leukocyte Antigen-E, α-Galactosidase A and α-Gal Antigenic Determinants Are Biased by Trichostatin A-Dependent Epigenetic Transformation of Triple-Transgenic Pig-Derived Dermal Fibroblast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marcin Samiec,Jerzy Wiater,Kamil Wartalski,Maria Skrzyszowska,Monika Trzcińska,Daniel Lipiński,Jacek Jura,Zdzisław Smorąg,Ryszard Słomski,Małgorzata Duda

International journal of molecular sciences 22: PubMed34575846

2021

Characterization of Mono- and Bi-Transgenic Pig-Derived Epidermal Keratinocytes Expressing Human and Genes-In Vitro Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Jerzy Wiater,Marcin Samiec,Kamil Wartalski,Zdzisław Smorąg,Jacek Jura,Ryszard Słomski,Maria Skrzyszowska,Marek Romek

International journal of molecular sciences 22: PubMed33573215

2021

Trichostatin A-Assisted Epigenomic Modulation Affects the Expression Profiles of Not Only Recombinant Human α1,2-Fucosyltransferase and α-Galactosidase A Enzymes But Also Galα1→3Gal Epitopes in Porcine Bi-Transgenic Adult Cutaneous Fibroblast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jerzy Wiater,Marcin Samiec,Maria Skrzyszowska,Daniel Lipiński

International journal of biological sciences 14:1521-1534 PubMed30263005

2018

Butein suppresses hepatocellular carcinoma growth via modulating Aurora B kinase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanfeng Zhou,Ming Li,Xinyou Yu,Ting Liu,Tian Li,Li Zhou,Wenbin Liu,Wei Li,Feng Gao
View all publications
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